Align L-rhamnose isomerase (EC 5.3.1.14) (characterized)
to candidate 3609048 Dshi_2437 L-rhamnose isomerase (RefSeq)
Query= reanno::Dino:3609048 (412 letters) >FitnessBrowser__Dino:3609048 Length = 412 Score = 836 bits (2160), Expect = 0.0 Identities = 412/412 (100%), Positives = 412/412 (100%) Query: 1 MGYENAKAAFAEWGVDTEAALARLKTIPISMHCWQGDDVVGFEQKTGSSGGGIQATGNHP 60 MGYENAKAAFAEWGVDTEAALARLKTIPISMHCWQGDDVVGFEQKTGSSGGGIQATGNHP Sbjct: 1 MGYENAKAAFAEWGVDTEAALARLKTIPISMHCWQGDDVVGFEQKTGSSGGGIQATGNHP 60 Query: 61 GRARTPDELRADLEFAYAMIPGRHRLNLHAMYLDTEATPDRDEIEIAHFAPWVDWARAQG 120 GRARTPDELRADLEFAYAMIPGRHRLNLHAMYLDTEATPDRDEIEIAHFAPWVDWARAQG Sbjct: 61 GRARTPDELRADLEFAYAMIPGRHRLNLHAMYLDTEATPDRDEIEIAHFAPWVDWARAQG 120 Query: 121 IGLDFNPTFFAHAKADDNLTLSHPDPGIRDFWIEHGKRSREIAAGMGAALGSACVNNIWV 180 IGLDFNPTFFAHAKADDNLTLSHPDPGIRDFWIEHGKRSREIAAGMGAALGSACVNNIWV Sbjct: 121 IGLDFNPTFFAHAKADDNLTLSHPDPGIRDFWIEHGKRSREIAAGMGAALGSACVNNIWV 180 Query: 181 PDGYKDTPVDRMAARARLEASLDAMLAPPQDRARMLDAVESKLFGIGVEACTVGSHEFYM 240 PDGYKDTPVDRMAARARLEASLDAMLAPPQDRARMLDAVESKLFGIGVEACTVGSHEFYM Sbjct: 181 PDGYKDTPVDRMAARARLEASLDAMLAPPQDRARMLDAVESKLFGIGVEACTVGSHEFYM 240 Query: 241 GYAIRKGTLLCLDMGHFHPTENIADKLSAVALSLDEILLHVSRPMRWDSDHVILLDDDIL 300 GYAIRKGTLLCLDMGHFHPTENIADKLSAVALSLDEILLHVSRPMRWDSDHVILLDDDIL Sbjct: 241 GYAIRKGTLLCLDMGHFHPTENIADKLSAVALSLDEILLHVSRPMRWDSDHVILLDDDIL 300 Query: 301 QMAQELVSADLLGRTRIGLDFFDATISRTAAWVIGTRNMQKALLRALLMPLDRLRAAEDA 360 QMAQELVSADLLGRTRIGLDFFDATISRTAAWVIGTRNMQKALLRALLMPLDRLRAAEDA Sbjct: 301 QMAQELVSADLLGRTRIGLDFFDATISRTAAWVIGTRNMQKALLRALLMPLDRLRAAEDA 360 Query: 361 LDFTTRLVVTEEVKDLPFGAVWAEFAAREDVPNGQKLIRELDRYQAQVSGRG 412 LDFTTRLVVTEEVKDLPFGAVWAEFAAREDVPNGQKLIRELDRYQAQVSGRG Sbjct: 361 LDFTTRLVVTEEVKDLPFGAVWAEFAAREDVPNGQKLIRELDRYQAQVSGRG 412 Lambda K H 0.321 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 787 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 412 Length of database: 412 Length adjustment: 31 Effective length of query: 381 Effective length of database: 381 Effective search space: 145161 Effective search space used: 145161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
Align candidate 3609048 Dshi_2437 (L-rhamnose isomerase (RefSeq))
to HMM TIGR01748 (rhaA: L-rhamnose isomerase (EC 5.3.1.14))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01748.hmm # target sequence database: /tmp/gapView.31716.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01748 [M=414] Accession: TIGR01748 Description: rhaA: L-rhamnose isomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-187 608.8 0.0 3.6e-187 608.5 0.0 1.0 1 lcl|FitnessBrowser__Dino:3609048 Dshi_2437 L-rhamnose isomerase ( Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dino:3609048 Dshi_2437 L-rhamnose isomerase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 608.5 0.0 3.6e-187 3.6e-187 5 410 .. 3 408 .. 1 411 [. 0.99 Alignments for each domain: == domain 1 score: 608.5 bits; conditional E-value: 3.6e-187 TIGR01748 5 yevakqryeavgidveealrqledvkislhcwqgddvegfenpegeltggiqatgnypgkartaeelrrdlekalsl 81 ye ak +++++g+d+e al +l+ ++is+hcwqgddv gfe++ g+ ggiqatgn+pg+art++elr+dle a+++ lcl|FitnessBrowser__Dino:3609048 3 YENAKAAFAEWGVDTEAALARLKTIPISMHCWQGDDVVGFEQKTGSSGGGIQATGNHPGRARTPDELRADLEFAYAM 79 99*************************************************************************** PP TIGR01748 82 ipgkkrlnlhaiyvetdekverdeikpkhfknwvewakeqglgldfnptlfshelaadgltlshadkeirefwiehc 158 ipg++rlnlha+y++t+ + +rdei+ hf +wv+wa++qg+gldfnpt+f+h +a d+ltlsh+d+ ir+fwieh+ lcl|FitnessBrowser__Dino:3609048 80 IPGRHRLNLHAMYLDTEATPDRDEIEIAHFAPWVDWARAQGIGLDFNPTFFAHAKADDNLTLSHPDPGIRDFWIEHG 156 ***************************************************************************** PP TIGR01748 159 kasrriseyfgkelgtpsvtniwipdglkdipvdrltprkrllealdevlsekldekytldavesklfglgaesytv 235 k+sr i++ +g lg+++v+niw+pdg+kd pvdr++ r rl ++ld +l+ d + ldavesklfg+g e+ tv lcl|FitnessBrowser__Dino:3609048 157 KRSREIAAGMGAALGSACVNNIWVPDGYKDTPVDRMAARARLEASLDAMLAPPQDRARMLDAVESKLFGIGVEACTV 233 ***************************************************************************** PP TIGR01748 236 gshefylgyaltrdklllldaghfhptevisnkisslllyskklllhvsrpvrwdsdhvvvlddelreiaseivrnd 312 gshefy+gya+ + +ll+ld ghfhpte i++k+s+++l +++llhvsrp+rwdsdhv++ldd++ ++a+e+v d lcl|FitnessBrowser__Dino:3609048 234 GSHEFYMGYAIRKGTLLCLDMGHFHPTENIADKLSAVALSLDEILLHVSRPMRWDSDHVILLDDDILQMAQELVSAD 310 ***************************************************************************** PP TIGR01748 313 llekvaigldffdasinriaawvigtrnmkkallkalleptadlkkleeegdyterlalleelkslpfgavwesyce 389 ll++++igldffda+i r aawvigtrnm+kall+all p l+ e + d+t+rl ++ee+k+lpfgavw + lcl|FitnessBrowser__Dino:3609048 311 LLGRTRIGLDFFDATISRTAAWVIGTRNMQKALLRALLMPLDRLRAAEDALDFTTRLVVTEEVKDLPFGAVWAEFAA 387 ***************************************************************************** PP TIGR01748 390 rkgvpvgeewlkevkayekev 410 r++vp g++ ++e+ y+ +v lcl|FitnessBrowser__Dino:3609048 388 REDVPNGQKLIRELDRYQAQV 408 *****************9998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (414 nodes) Target sequences: 1 (412 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.02 # Mc/sec: 8.22 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory