Align subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized)
to candidate 3610436 Dshi_3817 beta-ketoadipyl CoA thiolase (RefSeq)
Query= metacyc::MONOMER-3207 (400 letters) >FitnessBrowser__Dino:3610436 Length = 400 Score = 543 bits (1400), Expect = e-159 Identities = 272/399 (68%), Positives = 321/399 (80%), Gaps = 1/399 (0%) Query: 3 DVFICDAIRTPIGRFGGALAGVRADDLAAVPLKALIEPNPAVQWDQVDEVFFGCANQAGE 62 D ICD +RTPIGR+GGAL+ VRADDLAA+P+ AL+ NP V W +VDEV +G ANQAGE Sbjct: 2 DAVICDGVRTPIGRYGGALSSVRADDLAALPIAALMARNPGVDWARVDEVIYGAANQAGE 61 Query: 63 DNRNVARMALLLAGLPESIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMSR 122 DNRNVARMA LLAGLPE +PG+T+NRLCASGMDA+G A R I +GE +LAIAGG+ESMSR Sbjct: 62 DNRNVARMAALLAGLPEEVPGLTVNRLCASGMDAVGAAARGIKAGEYDLAIAGGIESMSR 121 Query: 123 APFVMGKAESGYSRNMKLEDTTIGWRFINPLMKSQYGVDSMPETADNVADDYQVSRADQD 182 APFVM KAES ++R + DTTIGWRF+NP + + +G+D+MP+TAD VA Y++SRADQD Sbjct: 122 APFVMPKAESAFTRAATVHDTTIGWRFVNPKIAAMHGIDTMPQTADTVAAAYEISRADQD 181 Query: 183 AFALRSQQKAAAAQAAGFFAEEIVPVRIAHK-KGETIVERDEHLRPETTLEALTKLKPVN 241 AFA RSQ + AAA AAG FA+EIVPV + + +V+RDEH RP T L LK +N Sbjct: 182 AFAARSQARWAAADAAGLFADEIVPVPVPQRGSAPILVDRDEHPRPGTDAARLAGLKGIN 241 Query: 242 GPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASGGVAPRVMGIGPVPA 301 GP +VTAGNASGVNDGAAAL++ASA A + HGLTP ARV+GMAS GVAPRVMGIGPVPA Sbjct: 242 GPGLSVTAGNASGVNDGAAALLIASAAAARAHGLTPMARVVGMASAGVAPRVMGIGPVPA 301 Query: 302 VRKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGVADDAPQVNPNGGAIALGHPLGMS 361 RKL +R G+ + DVIELNEAFASQ LA LR+LG+ADD +VNPNGGAIA+GHPLGMS Sbjct: 302 SRKLLDRAGLTLDQMDVIELNEAFASQSLATLRQLGLADDDVRVNPNGGAIAMGHPLGMS 361 Query: 362 GARLVLTALHQLEKSGGRKGLATMCVGVGQGLALAIERV 400 GARLVLTA HQL ++GGR L TMCVGVGQG AL +ERV Sbjct: 362 GARLVLTAAHQLRRTGGRYALCTMCVGVGQGTALILERV 400 Lambda K H 0.318 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 524 Number of extensions: 15 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 400 Length adjustment: 31 Effective length of query: 369 Effective length of database: 369 Effective search space: 136161 Effective search space used: 136161 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate 3610436 Dshi_3817 (beta-ketoadipyl CoA thiolase (RefSeq))
to HMM TIGR02430 (pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02430.hmm # target sequence database: /tmp/gapView.32704.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02430 [M=400] Accession: TIGR02430 Description: pcaF: 3-oxoadipyl-CoA thiolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.1e-207 674.9 15.8 2.3e-207 674.7 15.8 1.0 1 lcl|FitnessBrowser__Dino:3610436 Dshi_3817 beta-ketoadipyl CoA th Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Dino:3610436 Dshi_3817 beta-ketoadipyl CoA thiolase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 674.7 15.8 2.3e-207 2.3e-207 2 400 .] 2 400 .] 1 400 [] 1.00 Alignments for each domain: == domain 1 score: 674.7 bits; conditional E-value: 2.3e-207 TIGR02430 2 evyivdairtpiGrygGslssvraddlaavplkallarnpsldaaaiddvilGcanqaGednrnvarmaallaGlpv 78 ++ i+d++rtpiGrygG+lssvraddlaa+p++al+arnp +d+a +d+vi+G+anqaGednrnvarmaallaGlp+ lcl|FitnessBrowser__Dino:3610436 2 DAVICDGVRTPIGRYGGALSSVRADDLAALPIAALMARNPGVDWARVDEVIYGAANQAGEDNRNVARMAALLAGLPE 78 578************************************************************************** PP TIGR02430 79 svpgttvnrlcgsgldalglaaraikaGeadlviaGGvesmsrapfvlGkadsafsrsakledttiGwrfvnpklka 155 +vpg tvnrlc+sg+da+g aar+ikaGe dl iaGG+esmsrapfv+ ka+saf+r a ++dttiGwrfvnpk++a lcl|FitnessBrowser__Dino:3610436 79 EVPGLTVNRLCASGMDAVGAAARGIKAGEYDLAIAGGIESMSRAPFVMPKAESAFTRAATVHDTTIGWRFVNPKIAA 155 ***************************************************************************** PP TIGR02430 156 lyGvdsmpetaenvaeefgvsredqdafalrsqqrtaaaqakGffaeeivpveikqkkGeetvvdkdehlraettle 232 ++G+d+mp+ta+ va +++sr+dqdafa rsq+r+aaa+a+G+fa+eivpv ++q+ + vd+deh+r+ t lcl|FitnessBrowser__Dino:3610436 156 MHGIDTMPQTADTVAAAYEISRADQDAFAARSQARWAAADAAGLFADEIVPVPVPQRGSAPILVDRDEHPRPGTDAA 232 ***************************************************************************** PP TIGR02430 233 alaklkavvredgtvtaGnasGvndGaaalllaseeavkrhgltprarilaaasaGveprvmGlgpvpavkkllara 309 +la lk+++ ++ +vtaGnasGvndGaaall+as+ a+++hgltp ar++++asaGv+prvmG+gpvpa +kll+ra lcl|FitnessBrowser__Dino:3610436 233 RLAGLKGINGPGLSVTAGNASGVNDGAAALLIASAAAARAHGLTPMARVVGMASAGVAPRVMGIGPVPASRKLLDRA 309 ***************************************************************************** PP TIGR02430 310 glsledldvielneafaaqalavlrelgladddarvnpnGGaialGhplGasGarlvltalkqleksggryalatlc 386 gl+l+++dvielneafa+q+la+lr+lgladdd rvnpnGGaia+GhplG+sGarlvlta +ql+++ggryal+t+c lcl|FitnessBrowser__Dino:3610436 310 GLTLDQMDVIELNEAFASQSLATLRQLGLADDDVRVNPNGGAIAMGHPLGMSGARLVLTAAHQLRRTGGRYALCTMC 386 ***************************************************************************** PP TIGR02430 387 iGvGqGialvierv 400 +GvGqG al++erv lcl|FitnessBrowser__Dino:3610436 387 VGVGQGTALILERV 400 *************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (400 nodes) Target sequences: 1 (400 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 6.91 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory