Align 3-hydroxypropionate dehydrogenase (EC 1.1.1.59) (characterized)
to candidate 3607390 Dshi_0804 glucose-methanol-choline oxidoreductase (RefSeq)
Query= metacyc::MONOMER-15202 (579 letters) >FitnessBrowser__Dino:3607390 Length = 540 Score = 439 bits (1129), Expect = e-127 Identities = 244/544 (44%), Positives = 330/544 (60%), Gaps = 21/544 (3%) Query: 37 DYIVVGAGTAGCLLANRLSADPANRVLLIEAGGRDNYHWIHIPVGYLYCINNPRTDWRFR 96 DY+V+GAG+AGC++ANRLS D N+V+L+EAG D WIHIPVGY ++NP DW ++ Sbjct: 4 DYVVIGAGSAGCVVANRLSTDARNKVVLLEAGPPDTNPWIHIPVGYFKTMHNPTVDWCYK 63 Query: 97 TEPDPGLNGRSLIYPRGKTLGGCSSINGMLYLRGQARDYDGWAELTGDDAWRWDNCLPDF 156 T+ DPGLNGRS+ +PRGK LGG SS+NG+LY+RGQ DYD W ++ G+ W WD+ LP F Sbjct: 64 TQADPGLNGRSIDWPRGKVLGGSSSLNGLLYVRGQPEDYDRWRQM-GNAGWGWDDVLPLF 122 Query: 157 MRHEDHYRLDEGGDADPDHYKFHGHGGEWRIEKQRLKWQVLADFATAAVEAGVPRTRDFN 216 R E + R ADP +HG G + R++ + + AA G P D+N Sbjct: 123 RRAEANER-----GADP----WHGDDGPLAVSNMRIQRPICDAWVAAAQAMGYPFNPDYN 173 Query: 217 RGDNEGVDAFEVNQRSGWRWNASKAFLRGVEQRGNLTVWHSTQVLKLDFASGEGSEPRCC 276 EGV F++ R+G R +A+ A+L+ +R NL++ V +++ EG R Sbjct: 174 GASQEGVGYFQLTTRNGRRCSAAVAYLKPARKRPNLSIITRALVTRIEM---EGK--RVT 228 Query: 277 GVTVERAGKKVVT-TARCEVVLSAGAIGSPQLLQLSGIGPTALLAEHAIPVVADLPGVGE 335 GVT AG + T +AR EV+LS GAI SP +L LSGIG L H I LPGVG+ Sbjct: 229 GVTYTDAGGRAHTVSARREVILSGGAINSPHILMLSGIGDPDQLQAHGITPRHALPGVGK 288 Query: 336 NLQDHLQIRSIYKVKGAKTLNTMANSLIGKAKIGLEYILKRSGPMSMAPSQLCIFTRSSK 395 NLQDHLQ R ++K TLN SL+ +A+I L+Y L R+GPM+MA S F ++ Sbjct: 289 NLQDHLQARLVFKC-NEPTLNDEVRSLVNQARIALKYALFRAGPMTMAASLATGFLKTRP 347 Query: 396 EYEHPNLEYHVQPLSLEAFGQPLHDFPAITASVCNLNPTSRGTVRIKSGNPRQAPAISPN 455 + P++++HVQP S ++ G+ +H F A T SVC L P SRG +R+ +PR P I PN Sbjct: 348 DIATPDIQFHVQPWSADSPGEGVHPFSAFTMSVCQLRPESRGEIRLAGPDPRTYPTIHPN 407 Query: 456 YLSTEEDRQVAADSLRVTRHIASQPAFAKYDPEEFKPGVQYQSDEDLARL--AGDIGTTI 513 YLSTE D + +++ R IA A EEF+P D+D A L A +I Sbjct: 408 YLSTETDCATLTEGVKIARRIARADPLAGKIAEEFRPPANLALDDDAATLDWARSNSVSI 467 Query: 514 FHPVGTAKMGRDDDPMAVVDSHLRVRGVTGLRVVDASIMPTITSGNTNSPTLMIAEKAAG 573 +HP GT KMG P AVVD+ LRV G++GLRV D SIMP I SGNTN+P +MI EK + Sbjct: 468 YHPTGTCKMG--TGPGAVVDARLRVHGLSGLRVADCSIMPEIVSGNTNAPAIMIGEKLSD 525 Query: 574 WILK 577 +L+ Sbjct: 526 MVLE 529 Lambda K H 0.318 0.135 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 935 Number of extensions: 46 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 579 Length of database: 540 Length adjustment: 36 Effective length of query: 543 Effective length of database: 504 Effective search space: 273672 Effective search space used: 273672 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory