GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Dinoroseobacter shibae DFL-12

Align lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)
to candidate 3608018 Dshi_1425 aldehyde dehydrogenase (RefSeq)

Query= BRENDA::P25553
         (479 letters)



>lcl|FitnessBrowser__Dino:3608018 Dshi_1425 aldehyde dehydrogenase
           (RefSeq)
          Length = 484

 Score =  259 bits (662), Expect = 1e-73
 Identities = 150/471 (31%), Positives = 255/471 (54%), Gaps = 11/471 (2%)

Query: 4   PVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEAL 63
           P +H   I+G  V   G    +V NPAT+ V++  P+   +   +AI AA+ AQP W AL
Sbjct: 18  PYRHA--INGALVDSAGS--FEVFNPATDEVVAHAPNASRDQVEQAIAAAKAAQPGWAAL 73

Query: 64  PAIERASWLRKISAGIRERASEISALIVEEGGKIQQ-LAEVEVAFTADYIDYMAEWARRY 122
              ER +++   +  +     E+  L+  E GK +  +A  EV +   ++  +A+  RR 
Sbjct: 74  SQDERGAYIAAYADALDAHKQELITLLTTEQGKPRHSMATTEVEYAIFWVREVAK--RRL 131

Query: 123 EGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTP 182
           E E+I+ D P   + +    LGV   I PWNFP  L   K+AP L+TGNT+V+KPS +TP
Sbjct: 132 EDEVIE-DTPEHTVKVAHTPLGVVGAITPWNFPVLLGLWKIAPCLVTGNTMVMKPSPYTP 190

Query: 183 NNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAA 242
              + F +I  ++  P GV N+V G G   G  L  +P +A +S TGS + G K+MA+++
Sbjct: 191 LCTLRFGEIAQQV-FPAGVLNVVAG-GNEQGAWLTEHPDIAKISFTGSTATGRKVMASSS 248

Query: 243 KNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQFV 302
            N+ ++ LELGG  PAI++   D +  +  + D+   NSGQ C   +R+YV + +YD F+
Sbjct: 249 CNLKRITLELGGNDPAILLPGTDYKPLIPTLFDAAYGNSGQWCIAVKRLYVHESLYDDFL 308

Query: 303 NRLGEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGGKAVEGKG 362
                       GN  + N   +GP+ N     +++   A    +G  V  GG+  +G G
Sbjct: 309 RDFVAHAAEKTVGNGMDPNT-DLGPIQNKMQYGKLKDLFADVKAQGLSVPLGGEIPDGPG 367

Query: 363 YYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIYTQNLNV 422
            + P T++ +  ++  ++ EE FGP+LP++ +  L++ +  AND+++GL +S++  + + 
Sbjct: 368 NFVPITIVDNPPKDSRVVREEPFGPILPIIKWSDLDEVVRDANDTEFGLAASVWGPDRDT 427

Query: 423 AMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQ 473
           A+     L+ G  ++N  +   +     G ++SG+G  +G+ GL E+  T+
Sbjct: 428 AIGVANRLEAGTVWVNEIHIHGIDIPFGGHKQSGMGVENGQEGLKEFTNTK 478


Lambda     K      H
   0.318    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 543
Number of extensions: 26
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 479
Length of database: 484
Length adjustment: 34
Effective length of query: 445
Effective length of database: 450
Effective search space:   200250
Effective search space used:   200250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory