GapMind for catabolism of small carbon sources


Protein N515DRAFT_0039 in Dyella japonica UNC79MFTsu3.2

Annotation: FitnessBrowser__Dyella79:N515DRAFT_0039

Length: 344 amino acids

Source: Dyella79 in FitnessBrowser

Candidate for 6 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-threonine catabolism tdh hi L-threonine 3-dehydrogenase (EC (characterized) 65% 100% 476.9 L-gulonate 5-dehydrogenase (EC 34% 169.1
L-threonine catabolism tdh hi tdh: L-threonine 3-dehydrogenase (EC (TIGR00692) 100% 498.8 L-gulonate 5-dehydrogenase (EC 34% 169.1
D-sorbitol (glucitol) catabolism sdh lo L-iditol 2-dehydrogenase; EC (characterized) 33% 97% 189.9 L-threonine 3-dehydrogenase (EC 65% 476.9
xylitol catabolism xdhA lo L-iditol 2-dehydrogenase; EC (characterized) 33% 97% 189.9 L-threonine 3-dehydrogenase (EC 65% 476.9
D-xylose catabolism xdhA lo L-iditol 2-dehydrogenase; EC (characterized) 33% 97% 189.9 L-threonine 3-dehydrogenase (EC 65% 476.9
xylitol catabolism x5p-reductase lo Lmo2663 protein (characterized, see rationale) 32% 99% 159.5 L-threonine 3-dehydrogenase (EC 65% 476.9
D-mannitol catabolism mt2d lo mannitol 2-dehydrogenase (NADP+) (EC (characterized) 33% 85% 153.7 L-threonine 3-dehydrogenase (EC 65% 476.9

Sequence Analysis Tools

View N515DRAFT_0039 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory