GapMind for catabolism of small carbon sources

 

Protein N515DRAFT_0930 in Dyella japonica UNC79MFTsu3.2

Annotation: N515DRAFT_0930 methylglutaconyl-CoA hydratase

Length: 264 amino acids

Source: Dyella79 in FitnessBrowser

Candidate for 21 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-leucine catabolism liuC hi Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (characterized) 46% 96% 200.7 Isohexenylglutaconyl-CoA hydratase (EC 4.2.1.57) 39% 161.0
4-hydroxybenzoate catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-arginine catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-citrulline catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-lysine catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
phenylacetate catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-phenylalanine catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-proline catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-valine catabolism ech lo crotonase (EC 4.2.1.150) (characterized) 32% 98% 129 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
4-hydroxybenzoate catabolism paaF lo 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 35% 97% 128.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-isoleucine catabolism ech lo 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 35% 97% 128.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
phenylacetate catabolism paaF lo 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 35% 97% 128.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-phenylalanine catabolism paaF lo 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 35% 97% 128.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
4-hydroxybenzoate catabolism badK lo BadK (characterized) 36% 95% 125.9 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
phenylacetate catabolism badK lo BadK (characterized) 36% 95% 125.9 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-phenylalanine catabolism badK lo BadK (characterized) 36% 95% 125.9 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
phenylacetate catabolism paaG lo 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 34% 96% 117.5 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-phenylalanine catabolism paaG lo 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 34% 96% 117.5 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
4-hydroxybenzoate catabolism badI lo BadI (characterized) 31% 93% 98.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
phenylacetate catabolism badI lo BadI (characterized) 31% 93% 98.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7
L-phenylalanine catabolism badI lo BadI (characterized) 31% 93% 98.6 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 46% 200.7

Sequence Analysis Tools

View N515DRAFT_0930 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices: TMHMM

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MYTSLQLADRAGVRTVTMNRPQVHNAFDDSLIAELTDALAAAGRDENVRAVVLTGAGASF
SAGADLNWMRGMAKASEAENREDSLRLAALMRTLQFLPKPTVARVNGAAYGGGVGLIACC
DIAIGVDSAKFGLTEVKLGLVPAVISPYVIAAIGLRQSRRLFLTGELFDASTAQRIGLLH
QCVRAEELDDALAGVLAAFAKAGPVAQAQAKRLALRVAGQDQAQAERIDRENAELIARLR
VSAEGQEGLGAFLDKRAAAWTAQA

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory