Align BadK (characterized)
to candidate N515DRAFT_0930 N515DRAFT_0930 methylglutaconyl-CoA hydratase
Query= metacyc::MONOMER-943 (258 letters) >FitnessBrowser__Dyella79:N515DRAFT_0930 Length = 264 Score = 120 bits (301), Expect = 3e-32 Identities = 92/253 (36%), Positives = 136/253 (53%), Gaps = 14/253 (5%) Query: 14 VGIITLNRPDVLNALNDALMDALGGALLAFDADDGIGAIVIAGNTRAFAAGADI---ASM 70 V +T+NRP V NA +D+L+ L AL A D+ + A+V+ G +F+AGAD+ M Sbjct: 13 VRTVTMNRPQVHNAFDDSLIAELTDALAAAGRDENVRAVVLTGAGASFSAGADLNWMRGM 72 Query: 71 AAWSYSDVYGSNF-ITRNWETIRQIRKPVLAAVAGLAYGGGCELALACDIVIAGRSAKFA 129 A S ++ + + T++ + KP +A V G AYGGG L CDI I SAKF Sbjct: 73 AKASEAENREDSLRLAALMRTLQFLPKPTVARVNGAAYGGGVGLIACCDIAIGVDSAKFG 132 Query: 130 LPEIKLGLLPGAGGTQRLPRAIGKAKAMDMCLSARPLNAEEADRYGLVSRVVDDDRLRDE 189 L E+KLGL+P A + + AIG ++ + L+ +A A R GL+ + V + L D Sbjct: 133 LTEVKLGLVP-AVISPYVIAAIGLRQSRRLFLTGELFDASTAQRIGLLHQCVRAEELDD- 190 Query: 190 TVALATTIAAFSAPALMALKESLNRAF-----ESTLAEGILFERRELHARF-ASADAREG 243 ALA +AAF+ +A ++ A + AE I E EL AR SA+ +EG Sbjct: 191 --ALAGVLAAFAKAGPVAQAQAKRLALRVAGQDQAQAERIDRENAELIARLRVSAEGQEG 248 Query: 244 IQAFLEKRAPCFS 256 + AFL+KRA ++ Sbjct: 249 LGAFLDKRAAAWT 261 Lambda K H 0.321 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 120 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 264 Length adjustment: 25 Effective length of query: 233 Effective length of database: 239 Effective search space: 55687 Effective search space used: 55687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory