Aligments for a candidate for pcaF in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Aligments for a candidate for pcaF in Dyella japonica UNC79MFTsu3.2

Align subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized)
to candidate N515DRAFT_3009 N515DRAFT_3009 acetyl-CoA C-acetyltransferase

Query= metacyc::MONOMER-3207
         (400 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3009 N515DRAFT_3009
           acetyl-CoA C-acetyltransferase
          Length = 427

 Score =  178 bits (452), Expect = 2e-49
 Identities = 140/435 (32%), Positives = 216/435 (49%), Gaps = 54/435 (12%)

Query: 4   VFICDAIRTPIGRFGGALAGVRADDLAAVPLKALIEPNPAVQWDQVDEVFFGCANQAGED 63
           V +   IR P  R   A A V    ++   L AL+E    +  +++ EV  G   +   +
Sbjct: 8   VGVIGGIRIPFCRNNTAYADVGNFGMSVKVLGALVE-RFRLHGEELGEVAMGAVIKHSSE 66

Query: 64  NRNVARMALLLAGLPESIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMSRA 123
             N+AR A+L +GL  + PG+T  R C + +D        IA+G++E  IAGG ++ S  
Sbjct: 67  -WNLAREAVLSSGLAPTTPGITTARACGTSLDNAIIIANKIAAGQIEAGIAGGSDTTSDV 125

Query: 124 PFVMGKAESGYSRNMKLEDTTIGWR----------FINPLMKSQYGVD------SMPETA 167
           P V+G+    + + +   +   GW+           +  L  S  GV       SM +  
Sbjct: 126 PIVLGER---FRKRLLAINRAKGWQDKMAAFTRGFSLKELKPSFPGVAEPRTGMSMGDHC 182

Query: 168 DNVADDYQVSRADQDAFALRSQQKAAAAQAAGFFAEEIVPVRIAHKKGETIVERDEHLRP 227
           + +A ++ + R  QD  AL S QK AAA  AGFF + +VP R         ++RD  LR 
Sbjct: 183 ERMAKEWHIGREAQDRLALESHQKLAAAYEAGFFEDLVVPFR--------GLKRDGFLRA 234

Query: 228 ETTLEALTKLKP----VNGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLG 283
           ++++E L  LKP    ++G   T+TAGN++G++DGAAA++L S E   + GL  +A  L 
Sbjct: 235 DSSMEKLGTLKPAFDKISG-HGTLTAGNSTGLSDGAAAVLLGSDEWAARRGLKVQAYFLD 293

Query: 284 MASGGVAPRVMGIG----PVPAVRKLTERLGVAVSDFDVIELNEAFASQGLAVLR----- 334
            A       V G G    P  AV ++  R G+ + DFD  E++EAFA+Q L  LR     
Sbjct: 294 -AEVAAVDFVHGEGLLMAPTVAVPRMLARHGLTLQDFDFYEIHEAFAAQVLCTLRAWESE 352

Query: 335 -----ELGVAD-----DAPQVNPNGGAIALGHPLGMSGARLVLTALHQLEKSGGRKGLAT 384
                 LG+       D  ++N +G ++A GHP   +GAR+V T    LE+ G  +GL +
Sbjct: 353 TYCRNRLGLEQPLGSIDPAKLNVHGSSLAAGHPFAATGARIVATLAKMLEEKGSGRGLIS 412

Query: 385 MCVGVGQGLALAIER 399
           +C   G G+   +ER
Sbjct: 413 ICTAGGMGVTAILER 427


Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 396
Number of extensions: 17
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 427
Length adjustment: 31
Effective length of query: 369
Effective length of database: 396
Effective search space:   146124
Effective search space used:   146124
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources