Aligments for a candidate for yveA in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Aligments for a candidate for yveA in Dyella japonica UNC79MFTsu3.2

Align Aspartate-proton symporter; L-aspartate transporter (characterized)
to candidate N515DRAFT_4366 N515DRAFT_4366 Amino acid transporter

Query= SwissProt::O07002
         (520 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_4366 N515DRAFT_4366 Amino
           acid transporter
          Length = 554

 Score =  350 bits (899), Expect = e-101
 Identities = 193/525 (36%), Positives = 299/525 (56%), Gaps = 21/525 (4%)

Query: 1   MSKQGNFQKSMSLFDLILIGMGAIFGSAWLFAVSNVASKAGPSGAFSWILGGAIILLIGL 60
           MS     ++ +  F L+L G+G+I GS WLF     A  AGP   ++W+LG AII+ I L
Sbjct: 1   MSSSSPIRRDVGPFALMLTGLGSIIGSGWLFGAWRAAGLAGPGAIWAWVLGAAIIMTIAL 60

Query: 61  VYAELGAALPRTGGIIRYPVYSHGHLVGYLISFVTIVAYTSLISIEVTAVRQYVAYWFPG 120
            YAELGA  P +GG++RY  YSHG LVG++ ++   +A  S+I +E  A  QY+A W P 
Sbjct: 61  TYAELGAMFPESGGMVRYSHYSHGSLVGFIGAWANWIAIVSVIPVEAEASVQYMASW-PW 119

Query: 121 LTIK--------GSDSPTISGWILQFALLCLFFLLNYWSVKTFAKANFIISIFKYIVPIT 172
              +        G    +  G  +   L+ ++FLLN+WSVK FA++N  I+IFK IVP  
Sbjct: 120 KWAQDLYMHMPDGHGELSEPGLGIAAVLVIVYFLLNFWSVKLFARSNTAITIFKLIVPAA 179

Query: 173 IIIVLI---FHFQPENLSVQGFA-PFGFTGIQAAISTGGVMFAYLGLHPIVSVAGEVQNP 228
             I LI   FH +  ++ V G A       +  A++T G++F++ G    V++AGE  NP
Sbjct: 180 TGIALIASGFHSENFSVGVHGDAHAIDLAAVLTAVATAGIVFSFNGFQSPVNLAGEAHNP 239

Query: 229 KRNIPIALIICIIVSTIIYTVLQVTFIGAIPTETL-KHGWPAIGREFSLPFKDIAVMLGL 287
            ++IP A+I  I+++TI+Y +LQ+ ++G++P + L K GW  I  +F  PF ++A+++ L
Sbjct: 240 GKSIPFAVIGSIVLATIVYVILQLAYLGSVPPDLLAKAGWHGI--DFRSPFAELAIIVNL 297

Query: 288 GWLATLVILDAILSPGGNGNIFMNTTSRLVYAWARNGTLFGIFSKVNKDTGTPRASLWLS 347
            WLA L+ +DA +SP G G  +  TT+R++Y   RNGTL  I  +++   G PR ++W +
Sbjct: 298 HWLAMLLYVDAFISPSGTGITYTATTARMIYGMERNGTLPKILGRIHPKWGIPRPAMWFN 357

Query: 348 FALSIFWTLPFPSWNALVNVCSVALILSYAIAPISSAALRVNAKDLNRPFYLKGMSIIGP 407
            A+S  +   F  W  L  V SVA I+SY   P+S+  LR  A +L+RP  + G+ I+  
Sbjct: 358 LAVSYLFLFKFRGWGTLAAVISVATIISYLTGPVSAMTLRRTAPNLHRPLRIAGLPILAG 417

Query: 408 LSFIFTAFIVYWSGWKTVSWLLGSQLVMFLIYLCFSKYTPKEDVSLAQQLKSAWWLIGFY 467
           ++FI    ++YW+ W     ++   +V   +Y  +       D    +QLK AWWLI + 
Sbjct: 418 IAFIMATELLYWAKWPLTGEIILLMVVALPVYFFYQFKAGWHD--FGRQLKGAWWLIFYL 475

Query: 468 IMMLIFSYIGS--FGHGLGIISNPVDLILVAIGSLAIYYWAKYTG 510
             + + S+ GS  FG G G +S  +DL +VA+  L  Y W   +G
Sbjct: 476 PTLALVSWAGSTMFG-GKGYLSYGMDLAVVAVVGLVFYLWGVKSG 519


Lambda     K      H
   0.328    0.143    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 986
Number of extensions: 59
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 554
Length adjustment: 35
Effective length of query: 485
Effective length of database: 519
Effective search space:   251715
Effective search space used:   251715
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources