GapMind for catabolism of small carbon sources

 

D-cellobiose catabolism in Dyella japonica UNC79MFTsu3.2

Best path

bgl, MFS-glucose, glk

Also see fitness data for the top candidates

Rules

Overview: MetaCyc does not list any pathways for cellobiose utilization, but the major catabolic enzymes are believed to be intracellular cellobiase, periplasmic cellobiase, cellobiose-6-phosphate hydrolase, or cellobiose phosphorylase (PMID:28535986). These pathways all lead to glucose-6-phosphate, which is a central metabolic intermediate. There also may be a 3-ketoglucoside pathway in some Bacteroidetes, but this is not characterized.

73 steps (34 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
bgl cellobiase N515DRAFT_3248 N515DRAFT_3019
MFS-glucose glucose transporter, MFS superfamily N515DRAFT_1228 N515DRAFT_0382
glk glucokinase N515DRAFT_4210 N515DRAFT_0393
Alternative steps:
aglE' glucose ABC transporter, substrate-binding component (AglE)
aglF' glucose ABC transporter, permease component 1 (AglF) N515DRAFT_3134
aglG' glucose ABC transporter, permease component 2 (AglG) N515DRAFT_3133
aglK' glucose ABC transporter, ATPase component (AglK) N515DRAFT_4212 N515DRAFT_1562
ascB 6-phosphocellobiose hydrolase N515DRAFT_4211
bglF glucose PTS, enzyme II (BCA components, BglF)
bglG cellobiose PTS system, EII-BC or EII-BCA components
bglT cellobiose transporter BglT N515DRAFT_2416
cbp cellobiose phosphorylase
cbpB cellobiose ABC transporter, substrate-binding component CpbB
cbpC cellobiose ABC transporter, substrate-binding component CbpC
cbtA cellobiose ABC transporter, substrate-binding component CbtA
cbtB cellobiose ABC transporter, permease component 1 (CbtB)
cbtC cellobiose ABC transporter, permease component 2 (CbtC)
cbtD cellobiose ABC transporter, ATPase component 1 (CbtD) N515DRAFT_1085 N515DRAFT_1562
cbtF cellobiose ABC transporter, ATPase component 2 (CbtF) N515DRAFT_1085 N515DRAFT_1562
cdt cellobiose transporter cdt-1/cdt-2
cebE cellobiose ABC transporter, substrate-binding component CebE
cebF cellobiose ABC transporter, permease component 1 (CebF) N515DRAFT_3134
cebG cellobiose ABC transporter, permease component 2 (CebG) N515DRAFT_3133 N515DRAFT_3134
celEIIA cellobiose PTS system, EII-A component
celEIIB cellobiose PTS system, EII-B component
celEIIC cellobiose PTS system, EII-C component
crr glucose PTS, enzyme IIA
eda 2-keto-3-deoxygluconate 6-phosphate aldolase N515DRAFT_3178 N515DRAFT_1232
edd phosphogluconate dehydratase N515DRAFT_3177
gadh1 gluconate 2-dehydrogenase flavoprotein subunit
gadh2 gluconate 2-dehydrogenase cytochrome c subunit N515DRAFT_0703 N515DRAFT_2297
gadh3 gluconate 2-dehydrogenase subunit 3
gdh quinoprotein glucose dehydrogenase N515DRAFT_0461
glcS glucose ABC transporter, substrate-binding component (GlcS)
glcT glucose ABC transporter, permease component 1 (GlcT)
glcU glucose ABC transporter, permease component 2 (GlcU)
glcU' Glucose uptake protein GlcU
glcV glucose ABC transporter, ATPase component (GclV) N515DRAFT_4212 N515DRAFT_1562
gnl gluconolactonase N515DRAFT_1229
gtsA glucose ABC transporter, substrate-binding component (GtsA)
gtsB glucose ABC transporter, permease component 1 (GtsB)
gtsC glucose ABC transporter, permease component 2 (GtsC) N515DRAFT_3133
gtsD glucose ABC transporter, ATPase component (GtsD) N515DRAFT_4212 N515DRAFT_1562
kguD 2-keto-6-phosphogluconate reductase N515DRAFT_0108 N515DRAFT_3581
kguK 2-ketogluconokinase N515DRAFT_0916
kguT 2-ketogluconate transporter N515DRAFT_2085
manX glucose PTS, enzyme EIIAB
manY glucose PTS, enzyme EIIC
manZ glucose PTS, enzyme EIID
mglA glucose ABC transporter, ATP-binding component (MglA) N515DRAFT_3232 N515DRAFT_2413
mglB glucose ABC transporter, substrate-binding component N515DRAFT_3231
mglC glucose ABC transporter, permease component (MglC) N515DRAFT_3233 N515DRAFT_2415
msdB1 cellobiose ABC transporter, permease component 1 (MsdB1)
msdB2 cellobiose ABC transporter, permease component 2 (MsdB2)
msdC1 cellobiose ABC transporter, permease component 1 (MsdC1) N515DRAFT_3134
msdC2 cellobiose ABC transporter, permease component 1 (MsdC2) N515DRAFT_3133
msiK cellobiose ABC transporter, ATPase component N515DRAFT_4212 N515DRAFT_1562
PAST-A proton-associated sugar transporter A
pgmA alpha-phosphoglucomutase N515DRAFT_2990 N515DRAFT_1372
ptsG glucose PTS, enzyme IICB
ptsG-crr glucose PTS, enzyme II (CBA components, PtsG)
SemiSWEET Sugar transporter SemiSWEET
SMc04256 cellobiose ABC transporter, ATPase component N515DRAFT_4212 N515DRAFT_1562
SMc04257 cellobiose ABC transporter, permease component 1 N515DRAFT_3133
SMc04258 cellobiose ABC transporter, permease component 2
SMc04259 cellobiose ABC transporter, substrate-binding protein
SSS-glucose Sodium/glucose cotransporter
SWEET1 bidirectional sugar transporter SWEET1
TM0027 cellobiose ABC transporter, ATPase component 2 N515DRAFT_1085 N515DRAFT_2043
TM0028 cellobiose ABC transporter, ATPase component 1 N515DRAFT_1821
TM0029 cellobiose ABC transporter, permease component 2
TM0030 cellobiose ABC transporter, permease component 1
TM0031 cellobiose ABC transporter, substrate-binding component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory