GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gabT in Dyella japonica UNC79MFTsu3.2

Align 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 (characterized)
to candidate N515DRAFT_3308 N515DRAFT_3308 acetylornithine/N-succinyldiaminopimelate aminotransferase

Query= SwissProt::P22256
         (426 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3308 N515DRAFT_3308
           acetylornithine/N-succinyldiaminopimelate
           aminotransferase
          Length = 411

 Score =  221 bits (562), Expect = 4e-62
 Identities = 145/398 (36%), Positives = 204/398 (51%), Gaps = 35/398 (8%)

Query: 25  IFADRAENCRVWDVEGREYLDFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYE 84
           +  D  +  RVWD EGR+Y+D   GIAV   GH  P +V A+  Q +KL H+   V   E
Sbjct: 27  VVLDHGKGARVWDTEGRDYVDLGAGIAVNALGHQDPDLVDALVTQARKLWHSS-NVFYTE 85

Query: 85  PYLELCEIMNQKVPGDFAKKTLLVTTGSEAVENAVKIARAATKRSGT-------IAFSGA 137
           P L L E + Q     FA++  L  +G+EA E A+K+ R      G        + F G+
Sbjct: 86  PPLHLAEELVQA--SGFAERVFLCNSGTEANEAAIKLVRKWAASKGRAPEQRVILTFRGS 143

Query: 138 YHGRTHYTLALTGKVNP-YSAGMGLMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAA 196
           +HGRT    A+T    P Y      +PG  +R L         + + +A +   F    A
Sbjct: 144 FHGRT--LAAVTATAQPKYQENYEPLPGG-FRYL---------DFNDVAGLEAAF----A 187

Query: 197 PEDIAAIVIEPVQGEGGFYASSPAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQ 256
             D+AA+++EPVQGEGG   +SPAF++R R LCD H  +L+ DE+Q G GRTGTLFA  Q
Sbjct: 188 QGDVAAVMLEPVQGEGGVLPASPAFIRRARELCDTHEALLVLDEIQCGMGRTGTLFAHAQ 247

Query: 257 MGVAPDLTTFAKSIAGGFPLAGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFE 316
            GV PD+ T AK++  GFP+  +    +V + +  G  G T+ GNP+A   A   L+   
Sbjct: 248 DGVTPDIVTLAKALGCGFPIGAMLAGPKVAEVMQYGAHGTTFGGNPMAAAVARVALRKLA 307

Query: 317 QENLLQKANDLGQKLKDGLLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEI 376
              L+       Q L+DGL AI  +     +VRG G M+   L E             E+
Sbjct: 308 SAELMANVAKQAQALRDGLAAIDGELKLFAEVRGRGLMLGAVLAEAYKGR------AGEV 361

Query: 377 VARARDKGLILLSCGPYYNVLRILVPLTIEDAQIRQGL 414
           +  A   GL++L  GP  +VLR + PL I DA + +GL
Sbjct: 362 LDHAAAHGLLVLQAGP--DVLRFVPPLNITDADLAEGL 397


Lambda     K      H
   0.320    0.137    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 465
Number of extensions: 25
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 426
Length of database: 411
Length adjustment: 32
Effective length of query: 394
Effective length of database: 379
Effective search space:   149326
Effective search space used:   149326
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory