Align 2-deoxy-D-ribose dehydrogenase α subunit (characterized)
to candidate N515DRAFT_1294 N515DRAFT_1294 isoquinoline 1-oxidoreductase, alpha subunit
Query= metacyc::MONOMER-20832 (151 letters) >FitnessBrowser__Dyella79:N515DRAFT_1294 Length = 183 Score = 110 bits (276), Expect = 9e-30 Identities = 64/153 (41%), Positives = 88/153 (57%), Gaps = 6/153 (3%) Query: 1 MELRINQKAYQVDADADTPLLWVIRDDLGLTGTKYGCGLAQCGACSVLVDGNVVRSCVTP 60 M L +N ++ + AD DTPLL V+R +LGLTG + C +C AC+V V+ R+C P Sbjct: 28 MRLSVNGQSRAIQADPDTPLLRVLRQELGLTGAGFACTNGRCSACTVHVNDRPTRACRVP 87 Query: 61 VAGVVGREITTIEAIETDE---VGK--RVVATWVEHQVAQCGYCQSGQVMAATALLKHTP 115 V V G E+ TIE + E G+ V W+E+ V C CQSG +MAATALL P Sbjct: 88 VYLVQGTEVVTIEGLAAREGLPPGQLHAVQRAWIEYGVPPCP-CQSGMIMAATALLMDKP 146 Query: 116 APSKAQIDAAMINLCRCGTYNAIHAAVDDLAKQ 148 PS A+I+AA+ C CG + A+ LA++ Sbjct: 147 DPSPAEIEAALCLDCGCGARPHVLRAIRSLAER 179 Lambda K H 0.320 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 113 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 151 Length of database: 183 Length adjustment: 18 Effective length of query: 133 Effective length of database: 165 Effective search space: 21945 Effective search space used: 21945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory