Alignments for a candidate for drdehyd-beta in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Dyella japonica UNC79MFTsu3.2

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate N515DRAFT_4011 N515DRAFT_4011 isoquinoline 1-oxidoreductase, beta subunit

Query= metacyc::MONOMER-20833
         (739 letters)



>FitnessBrowser__Dyella79:N515DRAFT_4011
          Length = 769

 Score =  279 bits (713), Expect = 4e-79
 Identities = 234/774 (30%), Positives = 367/774 (47%), Gaps = 62/774 (8%)

Query: 9   DAPVNLSRRRFLASTA--VGALVIGFGLP-LGAGRVQAATSAERGTQVPAFLEIRPDGTV 65
           + P    RRRFL   A   GAL++G      G   V  A   +    + A++ I  DG V
Sbjct: 5   NTPALPQRRRFLQVLAGTAGALIVGVRYAEAGDAPVPPAMLGDTLYGLGAYVRIDADGNV 64

Query: 66  RLLSPFMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYVVMENGL---------R 116
            + +   + G G  TA+ +I+ +ELDAD A   V   P G   + +ENG          +
Sbjct: 65  LIGARDPDTGTGVATALPRILADELDADWAR--VSVVPLG---LGVENGNGEPRWIYGHQ 119

Query: 117 ITGGSMSVRMSYPTMRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGRSLGYG 176
           + G   S+  ++  +R+ GALAR +L+QA A++ GVP   L TQ G V+ +  GR   YG
Sbjct: 120 VGGTGSSIPAAWHDLRQAGALARWLLMQAAARRLGVPADRLRTQGGAVI-SPDGRRFDYG 178

Query: 177 ELASSALDMPVPDPASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDNMLHAA 236
            LA +A  +  P  A + ++ P ++  IG+P   +DA    TG+A ++ID    + L A 
Sbjct: 179 SLAEAASKV-APPTAPVPVKTPDRYTLIGQPAGDVDARAIVTGQAQFAIDNYFGDALVAV 237

Query: 237 VQHAPRLGMTVGSLRNQSQVEGMKGVHSV-------HVLPG------AVAVVAERWWHAK 283
           + H P +  T+   ++  + E +KGV  +        +LPG      +VAV+AE  W A 
Sbjct: 238 LVHCPWVDGTLAD-KDFHEAEAVKGVVKIVELKPEPGLLPGETPLAPSVAVLAEDTWSAL 296

Query: 284 RAVEAIQVDWQEAAADS----ALRVMPADFSSDKH--------REFLAAQQGPTRDDENE 331
           +A   ++++W+   + S    AL     D  + +         ++  A    PT    N+
Sbjct: 297 QARAKLKLEWKPGPSGSESSAALEQQANDLVNPREATAKGAAAKDAAARDNTPTMRVRND 356

Query: 332 GDVAGALKGAKTQVEATYHNQYLNHAQLEPPSALARFNPDGTLEIWLPNQAPDMFRADIA 391
           GDV G  K A  +V+ATY   +L HA  EP + LAR + D  + +  P QAP    + + 
Sbjct: 357 GDVDGMAKKAARRVDATYVQPWLAHATAEPMNCLARVDKDRAVLV-APTQAPQRAWSVVQ 415

Query: 392 KRTGLDPAQITLHSPLLGGFFGRHFLYDSANPYPQAIALAKAVSRPIKLIWSREEEFLRD 451
           + TG  P QI +  P +GG +GR   +D      +A+ LAK   RP++L+W+REE+   D
Sbjct: 416 RLTGFKPDQIEIRVPRVGGGYGRRLDHDFV---AEAVMLAKLADRPVRLLWTREEDLAHD 472

Query: 452 VLRPLAVVKFRAALDDKGLPVAIEAVSATEGPTEALAGKQGDKIDPTAVEGLSGKSYAIP 511
             RP +V +  A LD K   V  +   A+     A  G    ++  + V+     +  +P
Sbjct: 473 FYRPASVHRLSATLDRKRQIVTWDQRMASASAL-ARRGTPEQRLWTSEVDVNQLPAGLVP 531

Query: 512 NKRIAQIYVKGAPMLGYWRSVGNSLNDFFYESFLDELADKGGHDPYELRLHLLRDNPRLT 571
           N R     ++ A   G  R + +  N F  ESF+DE+A+    DP   RL LL +   + 
Sbjct: 532 NYRSDWYALQSALPRGPHRGMPHVSNAFAVESFVDEIANALKEDPLVTRLRLLGEAREVP 591

Query: 572 TLLQAAGELSGGWKRGPFTA-------EDGTRRARGVAMASPF--GSHTAAIAEVSIENG 622
             LQ  G    G                +  R   G+ MA     G++ A   E S++  
Sbjct: 592 --LQGGGSFDTGRLINVLKLVADRIEWRNWLRTVNGLGMACWHMDGAYVAHAIETSLQGE 649

Query: 623 KVKVHDIWQAIDPGSIVNPAIVEAQVNGAVALGLSQTLLEEAVYVDGKPRARNYDLYPIL 682
           K+ +  +  A+D G ++NP  +E Q+ GA    LS  L     + DG+P+ R+Y  YP+ 
Sbjct: 650 KLNIERVVCAVDVGRVINPLGLEGQIAGATLDALSAALNLAITFKDGQPQQRSYKDYPLA 709

Query: 683 APAQMA-RVHVRVVESGEKMGGIGEPPLPAVAPAVANAVAQLTGQRVRSLPLSR 735
           + AQ+   V V +V             +P  APA+ANAV +++  RVR LPL +
Sbjct: 710 SMAQLPDAVDVIIVPGDRDPAAASFLAMPTAAPALANAVFRVSAVRVRRLPLMK 763


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1448
Number of extensions: 70
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 769
Length adjustment: 40
Effective length of query: 699
Effective length of database: 729
Effective search space:   509571
Effective search space used:   509571
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources