Alignments for a candidate for fruI in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Dyella japonica UNC79MFTsu3.2

Align Fructose PTS system (E-I, HPr, and E-IIA components) (characterized)
to candidate N515DRAFT_3957 N515DRAFT_3957 phosphotransferase system, enzyme I, PtsI

Query= reanno::azobra:AZOBR_RS32325
         (846 letters)



>FitnessBrowser__Dyella79:N515DRAFT_3957
          Length = 576

 Score =  300 bits (768), Expect = 2e-85
 Identities = 210/579 (36%), Positives = 296/579 (51%), Gaps = 31/579 (5%)

Query: 281 VPGIAASPGLAIGPVHVLPRAAVSVPDEPVPLIEGG---DRLHEALSLTRQNLKALADDT 337
           +PG  AS G+A+G   ++  +   V   P+   E     ++LH AL   RQ L+ L    
Sbjct: 5   LPGSNASRGMALGRARLVQPSRYLVDTRPLGEDEVDVELEKLHRALDTARQELRELRGKL 64

Query: 338 ARRLGPSEAAIFAAQAEILNDTDLVTLACQLMVEGH---GVAWSWHQAVERTAAGLAALD 394
              L         A + +L+D +L+     L+  GH   G A    +  +R AA   A+D
Sbjct: 65  HGALAREVNEFIDAHSLLLDDQELLRGLDDLVRIGHYRPGAALKKQR--DRLAAVFEAMD 122

Query: 395 NPVLAARAADLRDVGQRVLARIDPALRTGGAPDLPDTPCILIAEDLSPSDTAALDMARVI 454
           +P L +R  D+  V  RV++ +                 ILIA+ ++P+D A L    ++
Sbjct: 123 DPYLRSRKEDVDQVINRVISALQRQTSREERKLAARVGEILIADTIAPADMAHLAGNGLL 182

Query: 455 GLATAQGGPTSHTAILARTLGLPAMVAGGAALMELANGTPAILDGQSGRLHLSPAAADIA 514
           G+  + G P SH+AILAR+L LP +V    AL  + +    +LD + G   + P A D+A
Sbjct: 183 GVVASSGSPYSHSAILARSLDLPMLVGVRDALATIHDDDLILLDAERGEAVVHPTAQDLA 242

Query: 515 DARAWIAREEARKAEEEARR-----GLPARTRDGHEVEIGANVNRPDQVAVALSQGAESV 569
             RAW      R+A++E RR       P RTRDG ++ + AN      +A+A ++GA+ V
Sbjct: 243 RYRAW-----QREAQQEGRRLASLANAPTRTRDGTDIRLLANAETTTDIAMARARGADGV 297

Query: 570 GLMRTEFLFLERGDAPGEDEQYETYRGMLTALEGRPLIVRALDIGGDK-QVPHLQLPHEE 628
           GL RTEFLFL     P EDEQ+  YR ++  + G P+ +R LD+G DK     L L  EE
Sbjct: 298 GLYRTEFLFLRHKGLPSEDEQFIAYRDLVLGMGGLPVTIRTLDLGADKADAAGLALRGEE 357

Query: 629 NPFLGVRGARLLLRRPELLETQLRALYRAAKDGGAKDGGALSIMFPMITALGEVQALRAA 688
           NP LGVRG RL LR P +  TQ+RA+ RAA  G  +      ++ PMIT   E+ A+R  
Sbjct: 358 NPALGVRGVRLSLRYPAVFTTQIRAILRAACYGPVR------VLVPMITQPDELIAVRTL 411

Query: 689 CERIRAEL-----DAP-AVPLGIMVEVPAAAIQADVLARHVDFFSIGTNDLTQYALAIDR 742
            +  R +L     D P  + LG M+EVPAAAI    L  H DF +IGTNDL QY LA DR
Sbjct: 412 FKLARQDLKRENVDLPEKLQLGAMIEVPAAAINVRSLLEHADFLAIGTNDLAQYVLASDR 471

Query: 743 QHPELAAEADSLHPAVLRLIRLTVEGAERHGRWVGVCGGIAGDPFGAALLTGLGVRELSM 802
            +  L    + L PA+LRLI   +    R G+ V +CG I GD    ALL  LG+ E SM
Sbjct: 472 GNDALDNIYNPLQPALLRLISYVITSGRRAGKPVSLCGEIGGDTKFTALLLALGLCEFSM 531

Query: 803 TPRDIPAVKDRLRGSDLSALKDAAQRALDCETADAVRAL 841
               +  V+DR+   +   L+  A   L C T +    L
Sbjct: 532 HSSQLLHVRDRIASLNHKGLRRLAPAILRCRTHEEAEDL 570


Lambda     K      H
   0.319    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1094
Number of extensions: 58
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 846
Length of database: 576
Length adjustment: 39
Effective length of query: 807
Effective length of database: 537
Effective search space:   433359
Effective search space used:   433359
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources