Aligments for a candidate for glcP in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Aligments for a candidate for glcP in Dyella japonica UNC79MFTsu3.2

Align Glucose/fructose:H+ symporter, GlcP (characterized)
to candidate N515DRAFT_0382 N515DRAFT_0382 MFS transporter, sugar porter (SP) family

Query= TCDB::P15729
         (468 letters)



>FitnessBrowser__Dyella79:N515DRAFT_0382
          Length = 472

 Score =  486 bits (1250), Expect = e-142
 Identities = 254/456 (55%), Positives = 332/456 (72%), Gaps = 6/456 (1%)

Query: 17  VLLISGVAALGGFLFGFDTAVINGAVAALQKHFQTDSLLTGLSVSLALLGSALGAFGAGP 76
           V+LI+  AALGGFLFGFDTAVINGAV A++  F   +   G +VS ALLGSALGA+ AGP
Sbjct: 17  VVLIAAAAALGGFLFGFDTAVINGAVDAVRGSFGLGAGRIGFAVSCALLGSALGAWYAGP 76

Query: 77  IADRHGRIKTMILAAVLFTLSSIGSGLPFTIWDFIFWRVLGGIGVGAASVIAPAYIAEVS 136
           +ADR GR++TM +AAVL  +S++GSGL   +WD + WR++GGIGVG ASVIAP YIAEVS
Sbjct: 77  LADRWGRVRTMQVAAVLLAISALGSGLVAGVWDLVLWRLVGGIGVGVASVIAPTYIAEVS 136

Query: 137 PAHLRGRLGSLQQLAIVSGIFIALLSNWFIALMAGGSAQNPWLFGAAAWRWMFWTELIPA 196
           PA +RGRLGSLQQLAIV GIF ALLS+ ++A  AGG++Q  WL G  AWRWMF   ++PA
Sbjct: 137 PARVRGRLGSLQQLAIVLGIFAALLSDAWLAGTAGGASQKLWL-GLEAWRWMFLVAVVPA 195

Query: 197 LLYGVCAFLIPESPRYLVAQGQGEKAAAILWKV----EGGDVPSRIEEIQATVSLDHKPR 252
           L+YG     +PESPR+LVA+G+ ++A  +L +V    +   +  ++ +I  ++  +++P 
Sbjct: 196 LIYGSLVLGVPESPRHLVAKGRMDEAKQVLRQVLDLQDEHALQHKLGDIAQSLRSEYRPG 255

Query: 253 FSDLLSRRGGLLPIVWIGMGLSALQQFVGINVIFYYSSVLWRSVGFTEEKSLLITVITGF 312
             DL     GLLP+VW+G+ LS  QQFVGINVIFYYSS LW SVGF+E  +  I+V+T  
Sbjct: 256 LRDLRGSMAGLLPVVWVGILLSVFQQFVGINVIFYYSSTLWHSVGFSESDAFSISVVTSV 315

Query: 313 INILTTLVAIAFVDKFGRKPLLLMGSIGMTITLGILSVVFGGATVVNGQPTLTGAAGIIA 372
           +N+L TLVAIA VD+ GRKPLL +GS GMT+TLG+++  F  A       +L    G++A
Sbjct: 316 VNVLVTLVAIALVDRIGRKPLLAIGSAGMTVTLGLMAWCFSQAAGSGAALSLPAPWGMVA 375

Query: 373 LVTANLYVFSFGFSWGPIVWVLLGEMFNNKIRAAALSVAAGVQWIANFIISTTFPPLLDT 432
           LV AN YV  FG SWGP+VWVLLGEMF N+IRA AL+VAA  QW+ANFII+++FP L + 
Sbjct: 376 LVAANAYVVFFGLSWGPMVWVLLGEMFPNRIRAIALAVAAAAQWVANFIITSSFPALSE- 434

Query: 433 VGLGPAYGLYATSAAISIFFIWFFVKETKGKTLEQM 468
           +GL  AYG+YA  A +S+ F+   V+ETKG  LE+M
Sbjct: 435 LGLSFAYGVYAFFALVSLVFVVKAVRETKGMELEEM 470


Lambda     K      H
   0.325    0.140    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 679
Number of extensions: 31
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 468
Length of database: 472
Length adjustment: 33
Effective length of query: 435
Effective length of database: 439
Effective search space:   190965
Effective search space used:   190965
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources