Alignments for a candidate for ytfR in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Alignments for a candidate for ytfR in Dyella japonica UNC79MFTsu3.2

Align galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized)
to candidate N515DRAFT_2413 N515DRAFT_2413 simple sugar transport system ATP-binding protein

Query= ecocyc::YTFR-MONOMER
         (500 letters)



>FitnessBrowser__Dyella79:N515DRAFT_2413
          Length = 505

 Score =  458 bits (1178), Expect = e-133
 Identities = 246/480 (51%), Positives = 329/480 (68%), Gaps = 4/480 (0%)

Query: 9   ILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGENGAGKSTLIKALTGVYHADRGTIWL 68
           +L+  GL K F    ALD VD +LR GE+ AL+G+NGAGKSTLIK LTGV   DRG++ L
Sbjct: 12  VLQARGLGKRFGATLALDGVDLALRAGEVHALMGQNGAGKSTLIKLLTGVERPDRGSVEL 71

Query: 69  EGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVADNLFIGREPKR--FGLLRRKEMEKRA 126
           +G+ I+P     AQ+ GIGTVYQEVNL PN+SVA+NL+ GR P+R    ++  +++   A
Sbjct: 72  DGRVIAPSTPMEAQRDGIGTVYQEVNLCPNLSVAENLYAGRYPRRRRLRMIDWRQVRDGA 131

Query: 127 TELMASYGFSLDVREPLNRFSVAMQQIVAICRAIDLSAKVLILDEPTASLDTQEVELLFD 186
             L+      LDV  PL  + VA++Q+VAI RA+ +SA+VLILDEPT+SLD  EV  LF 
Sbjct: 132 RSLLRQLHLELDVDAPLGSYPVAIRQMVAIARALGVSARVLILDEPTSSLDEGEVRELFR 191

Query: 187 LMRQLRDRGVSLIFVTHFLDQVYQVSDRITVLRNGSFVGCRETCELPQIELVKMMLGREL 246
           ++ QLR+RG++++FVTHFLDQVY VSDRITVLR+G  VG     +LP   LV  M+GR+L
Sbjct: 192 VIAQLRERGMAILFVTHFLDQVYAVSDRITVLRDGCRVGEYAVADLPPAALVNAMVGRDL 251

Query: 247 DTHALQRAGRTLLSDKPVAA-FKNYGKKGTIAPFDLEVRPGEIVGLAGLLGSGRTETAEV 305
            T A   A R    D P A   +  G +G + P DL+VR GE++GL GLLGSGRTE A +
Sbjct: 252 PTVAGADAERAPPPDAPPAIDAQGLGCRGKLHPVDLQVRRGEMLGLGGLLGSGRTELARL 311

Query: 306 IFGIKPADSGTALIKGKPQNLRSPHQASVLGIGFCPEDRKTDGIIAAASVRENIILALQA 365
           +FG+  A+ G   I G+   L+ P  A V G+  CPE+RKTDGI+A  SVRENI+LALQA
Sbjct: 312 LFGLDRAERGELRIGGERVELKHPADAVVRGLALCPEERKTDGIVAELSVRENIVLALQA 371

Query: 366 QRGWLRPISRKEQQEIAERFIRQLGIRTPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLI 425
           ++GW R +SR  Q E+A + ++ LGI+    E P+  LSGGNQQKV+L+RWL+T P+ LI
Sbjct: 372 RQGW-RGMSRARQDELARQLVQALGIKAADIETPVGLLSGGNQQKVMLARWLVTEPRLLI 430

Query: 426 LDEPTRGIDVGAHAEIIRLIETLCADGLALLVISSELEELVGYADRVIIMRDRKQVAEIP 485
           LDEPTRGIDV A  E++  +      G+A+L IS+E  EL  + DR+ +MR+R++  E+P
Sbjct: 431 LDEPTRGIDVAAKQELMAEVTRRAHAGMAVLFISAETGELTRWCDRIAVMRERRKAGELP 490



 Score = 95.9 bits (237), Expect = 3e-24
 Identities = 65/231 (28%), Positives = 119/231 (51%), Gaps = 4/231 (1%)

Query: 268 KNYGKKGTIAPFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLR 327
           K +G    +   DL +R GE+  L G  G+G++   +++ G++  D G+  + G+     
Sbjct: 20  KRFGATLALDGVDLALRAGEVHALMGQNGAGKSTLIKLLTGVERPDRGSVELDGRVIAPS 79

Query: 328 SPHQASVLGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIR 387
           +P +A   GIG   ++     +    SV EN+      +R  LR I  ++ ++ A   +R
Sbjct: 80  TPMEAQRDGIGTVYQEVN---LCPNLSVAENLYAGRYPRRRRLRMIDWRQVRDGARSLLR 136

Query: 388 QLGIRTPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIET 447
           QL +     + P+       +Q V ++R L    + LILDEPT  +D G   E+ R+I  
Sbjct: 137 QLHLEL-DVDAPLGSYPVAIRQMVAIARALGVSARVLILDEPTSSLDEGEVRELFRVIAQ 195

Query: 448 LCADGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAI 498
           L   G+A+L ++  L+++   +DR+ ++RD  +V E  +A+L   A++NA+
Sbjct: 196 LRERGMAILFVTHFLDQVYAVSDRITVLRDGCRVGEYAVADLPPAALVNAM 246


Lambda     K      H
   0.321    0.138    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 684
Number of extensions: 30
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 500
Length of database: 505
Length adjustment: 34
Effective length of query: 466
Effective length of database: 471
Effective search space:   219486
Effective search space used:   219486
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources