GapMind for catabolism of small carbon sources

 

Alignments for a candidate for AO353_21725 in Dyella japonica UNC79MFTsu3.2

Align ABC transporter for D-glucosamine, ATPase component (characterized)
to candidate N515DRAFT_2043 N515DRAFT_2043 putative ABC transport system ATP-binding protein

Query= reanno::pseudo3_N2E3:AO353_21725
         (265 letters)



>FitnessBrowser__Dyella79:N515DRAFT_2043
          Length = 230

 Score =  133 bits (335), Expect = 3e-36
 Identities = 93/237 (39%), Positives = 137/237 (57%), Gaps = 25/237 (10%)

Query: 15  LLEIRDLHKQYG----PLEVLKGVDLTMQRGNVVTLIGSSGSGKTTLLRCVNMLEEFQGG 70
           L+E+RDL K Y      +EVL  ++L +  G+ + L+G SGSGKTTLL  +  L+   GG
Sbjct: 6   LIEVRDLSKVYERGKQKVEVLHHINLNIAEGDFLALMGPSGSGKTTLLNLIGGLDSPTGG 65

Query: 71  QILLDGESIGYHEVNGKRVRHSEKVIAQHRAMT-GMAFQQFNLFPHLTALQNVTLGLLKV 129
            I + G+ I          +     +A+ RA   G  FQ +NL P LTA +NV L LL  
Sbjct: 66  SIGVGGQRID---------QLGAGALAKWRAANVGFVFQFYNLMPMLTAQRNVELPLLLT 116

Query: 130 K----KLHKDEAVVLAEKWLERVGLLERRDHYPGQLSGGQQQRVAIARAIAMNPSLMLFD 185
           K    +  K+ A+ L     + VGL ER  H P +LSGGQQQRVAIARAI  +P+L++ D
Sbjct: 117 KLSAAQRRKNAAIAL-----QLVGLDERSSHKPSELSGGQQQRVAIARAIVSDPTLLVCD 171

Query: 186 EVTSALDPELVGEVLSVIKGL-AEDGMTMLLVTHEMRFAFEVSDKIVFMNQGRIEEQ 241
           E T  LD +   +VL +++ L  E G T+++VTH+ + A E ++  + +++G + EQ
Sbjct: 172 EPTGDLDRQSAEDVLGLLRTLNREHGKTIVMVTHDPK-AAEYANHTLHLDKGTLVEQ 227


Lambda     K      H
   0.319    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 133
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 265
Length of database: 230
Length adjustment: 24
Effective length of query: 241
Effective length of database: 206
Effective search space:    49646
Effective search space used:    49646
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory