GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aglK' in Dyella japonica UNC79MFTsu3.2

Align Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale)
to candidate N515DRAFT_1562 N515DRAFT_1562 sulfate transport system ATP-binding protein

Query= uniprot:A8LLL2
         (373 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_1562 N515DRAFT_1562 sulfate
           transport system ATP-binding protein
          Length = 384

 Score =  187 bits (475), Expect = 4e-52
 Identities = 114/296 (38%), Positives = 172/296 (58%), Gaps = 16/296 (5%)

Query: 4   LKLTGVEKAYGDVKVLSNINLDIQQGELIVFVGPSGCGKSTLLRMIAGLEKITGGTLEID 63
           L +  + + YG    L + +LDI +GE +  +GPSG GKS+LLR++AGL+    G +  D
Sbjct: 3   LSIRQLTRRYGAFAALDDFSLDIAEGEFVALLGPSGSGKSSLLRILAGLDDPDRGDVLRD 62

Query: 64  GTVVNDVPPAQRGIAMVFQSYALYPHMTVRENMSFALKI----AKKSQAEIDAAVEAAAE 119
           GT +  +P  +R I +VFQ YAL+PHMTV +N++F L++     + S+ +I A VE    
Sbjct: 63  GTDLLALPAQRRDIGLVFQHYALFPHMTVADNIAFGLRVRPRARRPSRRDIAARVEDLLR 122

Query: 120 KLQLGQYLDRLPKALSGGQRQRVAIGRSIVRDPKVYLFDEPLSNLDAALRVATRLEIAQL 179
           ++QL +   R P  LSGGQRQRVA+ R++  +P + L DEP   LDA +R   R+ +  L
Sbjct: 123 RVQLEELGRRYPTQLSGGQRQRVALARALAVEPSLLLLDEPFGALDAQVRGTLRVWLRDL 182

Query: 180 KEAMPESTMVYVTHDQVEAMTLATRIVVLAGGGIAQVGSPLELYEKPENEFVAQFIGSPK 239
           + ++  +T V VTHDQ EA+ LA R+VV+  G I QVG+P E+Y +P   FV  F+G  +
Sbjct: 183 QRSLGLTT-VLVTHDQDEALELADRVVVMNRGRIEQVGAPSEIYREPATPFVHGFVG--R 239

Query: 240 MNLLPGKIIGTGAQTTVEMTDGGRAV-SDYPSDDSLMGAAVNVGVRPEDMVEAAPG 294
            N + G +          +  GG +   + P D  L G  +   +RPE +  A+ G
Sbjct: 240 ANRIRGHV------ERDRLHLGGHSFQGELPGD--LAGREIEAWLRPEHLALASRG 287


Lambda     K      H
   0.316    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 350
Number of extensions: 15
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 373
Length of database: 384
Length adjustment: 30
Effective length of query: 343
Effective length of database: 354
Effective search space:   121422
Effective search space used:   121422
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory