GapMind for catabolism of small carbon sources

 

Alignments for a candidate for BPHYT_RS24015 in Dyella japonica UNC79MFTsu3.2

Align ABC transporter related (characterized, see rationale)
to candidate N515DRAFT_1085 N515DRAFT_1085 D-methionine transport system ATP-binding protein

Query= uniprot:B2TBJ9
         (263 letters)



>FitnessBrowser__Dyella79:N515DRAFT_1085
          Length = 336

 Score =  163 bits (412), Expect = 5e-45
 Identities = 101/249 (40%), Positives = 144/249 (57%), Gaps = 17/249 (6%)

Query: 13  NIHKSFG----DHHVLKGISLDAHQGDVISILGASGSGKSTFLRCLNLLETPDDGSVSLA 68
           ++HKS+     D   L+  SLD   G+V  I+G SG+GKST +R +NLLE P  GS+ + 
Sbjct: 6   DVHKSYRVDGKDIPALQPFSLDIADGEVFGIIGHSGAGKSTLIRLINLLERPSGGSILID 65

Query: 69  GEELKMKRRGDGKLQPSDRRQVDRVRSQLGMVFQNFNLWSHMTVLENLIEGPMRVQKRSR 128
           G E  M   GD  L+   RR        +GM+FQ+FNL S  TV +N I  P+R+   + 
Sbjct: 66  GTE--MTALGDAALRAQRRR--------IGMIFQHFNLLSSQTVADN-IAFPLRLAGETD 114

Query: 129 AESVE-EAEALLAKVGLAEKRGHYPAHLSGGQQQRVAIARALAMHPKVMLFDEPTSALDP 187
           A  ++   + LL +VGL      YPA LSGGQ+QRV IARALA  P ++L DE TSALDP
Sbjct: 115 AGKIKARVDELLRRVGLEAHASKYPAQLSGGQKQRVGIARALANRPSILLCDEATSALDP 174

Query: 188 ELVGEVLRVMRSLAEEGR-TMLVVTHEMGFARHVSNRVMFLHQGQVEADGTPDEVFVECK 246
           +    VL ++  +  E + T++++THEM   R V +RV  L  G++   G   +VF+  +
Sbjct: 175 QTTASVLELLAEINRELKLTIVLITHEMDVVRRVCDRVAVLDAGRIVEHGAVADVFLHPR 234

Query: 247 SDRFRQFVS 255
               R+FV+
Sbjct: 235 HPTTRRFVN 243


Lambda     K      H
   0.318    0.133    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 210
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 263
Length of database: 336
Length adjustment: 26
Effective length of query: 237
Effective length of database: 310
Effective search space:    73470
Effective search space used:    73470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory