Alignments for a candidate for Ga0059261_1577 in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Alignments for a candidate for Ga0059261_1577 in Dyella japonica UNC79MFTsu3.2

Align L-glutamine and L-histidine transporter (characterized)
to candidate N515DRAFT_0606 N515DRAFT_0606 basic amino acid/polyamine antiporter, APA family

Query= reanno::Korea:Ga0059261_1577
         (470 letters)



>FitnessBrowser__Dyella79:N515DRAFT_0606
          Length = 477

 Score =  315 bits (807), Expect = 2e-90
 Identities = 180/438 (41%), Positives = 261/438 (59%), Gaps = 15/438 (3%)

Query: 32  LVALGVGAIVGTGILTLIGVGAG-KAGPAVIMSFVIAGAICACAALAYAEMATMMPASGS 90
           L A+G+G I+G G+  L G  A   AGPAV +SF+IAG   A AAL YAE A ++P +GS
Sbjct: 35  LTAIGIGGIIGVGVFVLAGQQAALNAGPAVAISFLIAGIASAAAALCYAEFAGLIPVTGS 94

Query: 91  AYAYSYAVLGEIIAWVVGWSLILEYSLVVSTVAVGWSGYAAPLLHAWTGMPLELMA---- 146
           AY Y YAVLGE+ AW++GW L+LEY+L+V+ VA+GWSGY    L A  G+ L + A    
Sbjct: 95  AYTYGYAVLGELAAWLIGWDLLLEYALIVAVVAIGWSGYVQSAL-ASIGVDLPVWAKGAI 153

Query: 147 GPHANGIVNLPAIFIIAVVAGLLCLGTKESATLNAALVVVKIIALAVFVAVALPYFNGAN 206
           G     + N+ A  +   V+ LL   T+  A  N  +V +K+ A+A+ + V   Y    N
Sbjct: 154 GTGEGHVFNVVAALVTLGVSALLIFRTEWGARFNTFVVAIKVAAVALVIGVGAFYVKPEN 213

Query: 207 LEPFAPFGFAKTISPDGVE----RGVMAAAAIIFFAFYGFDAISTAAEETKNPGRDLAIG 262
             PF P    + +  DGV     +GV  AAA++FFA +G+D ++TAAEE+KNP RDL   
Sbjct: 214 WVPFIP---ERIVGSDGVGHFGFQGVATAAAVVFFAVFGYDTLTTAAEESKNPQRDLPRA 270

Query: 263 IVGSMIACVAIYMLVAVAAVGATPFTHFANSPEPLALILRDLGRPGFATFLAVSAIIALP 322
           ++ S+   +A+Y+ V++   G   +T    +  P+A   + LG    A  ++VSA+  L 
Sbjct: 271 VLLSLGISMAMYLAVSMVLTGIAHYTTL-KTDAPVADAFKGLGLHWVALTVSVSAVFGLI 329

Query: 323 TVLLGFLFGQSRIFFTMARDGMLPIGLAKVSKR-GSPVRITLFTAAIVAVIAGLLPIDEI 381
           +VL  F+ G +RI+F +ARDG+LP   AK   R G+P R T+      A++AGLLPI+E+
Sbjct: 330 SVLFAFMLGATRIWFALARDGLLPGWFAKPHPRYGTPHRPTIVLGVFTALVAGLLPIEEV 389

Query: 382 AALANAGTLAAFTAVAVCMMVLRVRAPDMPRMFRTPLWWLVGAIAVLGCIYLFFSLPVKT 441
           A L N G L+AF  +   +++LR R PD+ R FRTPL  L+  + +   I+L   LP  T
Sbjct: 390 AKLVNIGVLSAFIVICSSVLILRKRKPDLYRAFRTPLVPLIPLVGIGFSIWLLAELPWVT 449

Query: 442 QLWFLAWNALGVVIYFAY 459
              FL W ++G+V+YF Y
Sbjct: 450 WEVFLIWVSIGLVVYFGY 467


Lambda     K      H
   0.327    0.140    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 715
Number of extensions: 49
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 470
Length of database: 477
Length adjustment: 33
Effective length of query: 437
Effective length of database: 444
Effective search space:   194028
Effective search space used:   194028
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources