Aligments for a candidate for Ga0059261_1577 in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Aligments for a candidate for Ga0059261_1577 in Dyella japonica UNC79MFTsu3.2

Align L-glutamine and L-histidine transporter (characterized)
to candidate N515DRAFT_2924 N515DRAFT_2924 amino acid/polyamine/organocation transporter, APC superfamily (TC 2.A.3)

Query= reanno::Korea:Ga0059261_1577
         (470 letters)



>FitnessBrowser__Dyella79:N515DRAFT_2924
          Length = 492

 Score =  347 bits (890), Expect = e-100
 Identities = 200/488 (40%), Positives = 278/488 (56%), Gaps = 37/488 (7%)

Query: 1   MAGGLFRTKRVKDAAEQAP------EHRLAATLSWPHLVALGVGAIVGTGILTLIGVGAG 54
           M   LF T ++  A+   P      E  L   L+  HLV LG+GAI+G GI  + G  A 
Sbjct: 1   MLKNLFATTQISPASADLPGGGAHGEATLKRALTARHLVLLGIGAIIGAGIFVITGQAAA 60

Query: 55  K-AGPAVIMSFVIAGAICACAALAYAEMATMMPASGSAYAYSYAVLGEIIAWVVGWSLIL 113
           + AGPA+++SFV AG  CA AAL YAE A M+P SGSAY+YSYA LGE +AW VGWSL+L
Sbjct: 61  EHAGPAIVLSFVFAGIACALAALCYAEFAAMLPVSGSAYSYSYATLGEYVAWFVGWSLVL 120

Query: 114 EYSLVVSTVAVGWSGY---AAPLLHAWTG----MPLELMAGP--------HANGI-VNLP 157
           EY   V+TVA GWSGY      L+  W G    +P  L A P         A G+ +NLP
Sbjct: 121 EYLFTVATVAAGWSGYFNKLLALISGWIGHDVSLPQTLAAAPFTVVDGHIQATGMFINLP 180

Query: 158 AIFIIAVVAGLLCLGTKESATLNAALVVVKIIALAVFVAVALPYFNGANLEPFAPFGFAK 217
           A+ IIA + GL  +G  +SA +N+ +V +K+  + +F+A A  Y N  N  PF P     
Sbjct: 181 AVAIIAAITGLCYVGITQSAFVNSIIVAIKVTVILLFIAFATKYINPDNWHPFIP----- 235

Query: 218 TISPDGVER----GVMAAAAIIFFAFYGFDAISTAAEETKNPGRDLAIGIVGSMIACVAI 273
             + +G  +    GV  AAAI+FF++ GFDA+STAA E KNP RD+ IGI+GS+I C  +
Sbjct: 236 --ASEGASKYGWAGVGRAAAIVFFSYIGFDAVSTAAGEAKNPQRDMPIGIIGSLILCTIL 293

Query: 274 YMLVAVAAVGATPFTHFANSPEPLALILRDLGRPGFATFLAV-SAIIALPTVLLGFLFGQ 332
           Y++VA    G   F     +PEP++  L +     +   + V  A+  L +V+L  L GQ
Sbjct: 294 YIIVAGILTGIADF-RLLGTPEPVSTALDNYPSLHWLQIIVVIGAVTGLSSVMLVMLMGQ 352

Query: 333 SRIFFTMARDGMLPIGLAKV-SKRGSPVRITLFTAAIVAVIAGLLPIDEIAALANAGTLA 391
            RIF++MARDG++P    ++  K  +P   T+    + A + GL  I  +  +   GTL 
Sbjct: 353 PRIFYSMARDGLIPAVFGRIHQKFRTPHVGTVVVGVLAAALGGLFNIGVLGEMVAMGTLL 412

Query: 392 AFTAVAVCMMVLRVRAPDMPRMFRTPLWWLVGAIAVLGCIYLFFSLPVKTQLWFLAWNAL 451
           AF  V + ++VLR   P++PR FR P+ W+V  +  L C+ LF    ++   W LAW A+
Sbjct: 413 AFATVCIGVLVLRYTRPELPRAFRVPVPWIVCPLGALACMALFLQSFLEHWRWMLAWIAI 472

Query: 452 GVVIYFAY 459
           G  IYF Y
Sbjct: 473 GQAIYFLY 480


Lambda     K      H
   0.327    0.140    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 687
Number of extensions: 39
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 470
Length of database: 492
Length adjustment: 34
Effective length of query: 436
Effective length of database: 458
Effective search space:   199688
Effective search space used:   199688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources