GapMind for catabolism of small carbon sources

 

Aligments for a candidate for acdH in Dyella japonica UNC79MFTsu3.2

Align 2-methylbutanoyl-CoA dehydrogenase / butanoyl-CoA dehydrogenase / isobutyryl-CoA dehydrogenase (EC 1.3.8.1; EC 1.3.8.5) (characterized)
to candidate N515DRAFT_0492 N515DRAFT_0492 hypothetical protein

Query= reanno::pseudo3_N2E3:AO353_25680
         (375 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_0492 N515DRAFT_0492
           hypothetical protein
          Length = 386

 Score =  338 bits (867), Expect = 1e-97
 Identities = 180/376 (47%), Positives = 253/376 (67%), Gaps = 7/376 (1%)

Query: 4   TDEQLQISDAARQFAQERLKPFAAEWDREHRFPKEAIGEMAELGFFGMLVPEQWGGCDTG 63
           T++QL I   AR FAQ+R+ P AAE D +  FP E I EM +LG  G+ VP ++GG    
Sbjct: 6   TEDQLSIQSIARDFAQKRIVPVAAELDAKGEFPLENIREMGQLGLMGIEVPHEYGGAGMD 65

Query: 64  YLAYAMALEEIAAGDGACSTIMSVHNSVGCVPILKFGNDDQKERFLKPLASGAMLGAFAL 123
            +AY +A+ EIAA D A ST+MSV+NS+ C  ILK GN++QK+++++ +A G  +GA+AL
Sbjct: 66  PIAYVLAMIEIAAADAATSTVMSVNNSLFCNGILKHGNEEQKQKYVRAIAQGEAIGAYAL 125

Query: 124 TEPQAGSDASSLKTRARLNGD-HYVLNGCKQFITSGQNAGVVIVFAVTDPSAGKRGISAF 182
           TEPQ+GSDAS++ TRA  N D  +V+NG K +ITSG  A  +++FA++ P  G RG+SAF
Sbjct: 126 TEPQSGSDASAMHTRATKNADGDWVINGKKSWITSGPVARYIVLFAISTPGIGARGVSAF 185

Query: 183 IVPTDSPGYKVARVEDKLGQHASDTCQILFEDVQVPVANRLGEEGEGYKIALANLEGGRV 242
           I+ T  PG+   + E KLG  AS TC+I F D   P  N LGEEG+G+ IA+  L+ GR+
Sbjct: 186 IIDTQLPGFAAGKTEPKLGIRASATCEIEFSDYVCPKENLLGEEGKGFSIAMGVLDAGRI 245

Query: 243 GIASQSVGMARAAFEAARDYARERESFGKPIIEHQAVAFRLADMATQIAVARQMVHYAA- 301
           GIASQSVG+ARAA+EA   ++R+R++FG+ I   Q    ++ADM  ++  A  +   AA 
Sbjct: 246 GIASQSVGIARAAYEATLQWSRDRKAFGQAIGSFQMTQAKIADMKCKLDAATLLTLRAAW 305

Query: 302 ----ALRDSGKPALVEASMAKLFASEMAEKVCSTALQTLGGYGYLSDFPLERIYRDVRVC 357
               A ++ G+    EAS+AKL ASE A  +   A+Q  GG GY  + PLER +RD ++ 
Sbjct: 306 TKGQAEKNGGRFG-TEASVAKLVASEAAMWIAHQAVQIHGGMGYSKEMPLERYFRDAKIT 364

Query: 358 QIYEGTSDIQRMVISR 373
           +IYEGTS+IQR+VI+R
Sbjct: 365 EIYEGTSEIQRLVIAR 380


Lambda     K      H
   0.319    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 341
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 386
Length adjustment: 30
Effective length of query: 345
Effective length of database: 356
Effective search space:   122820
Effective search space used:   122820
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory