Align fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 (characterized)
to candidate N515DRAFT_0416 N515DRAFT_0416 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase
Query= CharProtDB::CH_003230 (714 letters) >FitnessBrowser__Dyella79:N515DRAFT_0416 Length = 686 Score = 377 bits (968), Expect = e-108 Identities = 253/682 (37%), Positives = 362/682 (53%), Gaps = 35/682 (5%) Query: 16 IAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIG 75 I +T+D +N L E ++ I+++L K GV+ SAKP F GADI Sbjct: 19 IVTLTLDRANSSVNALSREVLDELGQIVERLSIEKPA-GVLIHSAKPGGFAVGADIKEF- 76 Query: 76 NCKTAQEAEALAR--QGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPK 133 + A++ L GQ++ + LP +AAIHGAC+GGG EL LAC R+ DD K Sbjct: 77 -VEYARDGTVLQNIENGQRVFESLARLPCPTVAAIHGACMGGGTELVLACRQRIAADDEK 135 Query: 134 TVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSI 193 T +GLPEV LG+ PG GGT RLPRLIG AL ++LTGK L A++A LG+VD + P + Sbjct: 136 TRIGLPEVMLGIHPGWGGTARLPRLIGALDALPVMLTGKALSARRAAALGVVDRLAPPNE 195 Query: 194 LLEAAVELAKKERPSSRPLPVRERILAGPL--GRALLFKMVGKKTEHKTQG-NYPATERI 250 LL A L + RP +RP R + A R +L MV K+T K + +YPA + Sbjct: 196 LLAEARALLR--RPHARPAARRLKAWATNTWPARQILAPMVLKQTAAKVRKEHYPAPFAL 253 Query: 251 LEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNS 310 + V + G EAR+ +LA T ++ L IF +K GS + Sbjct: 254 INVWQRG-GSSIQQRLKLEARSVAKLAETSTARNLIRIFLLQERLKGQ-GSGVEHG-IEH 310 Query: 311 VGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASER 370 V ++G G+MGG IA A K G V ++D + I AL + E K LK E+ Sbjct: 311 VHVVGAGVMGGDIAAWAAFK-GFQVTLQDREMKFIQPALDRARALYEKK-----LKTPEK 364 Query: 371 DKQLAL-ISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPI 429 ++ A + + +G A DL IEA+FEN E KQ + A +E A I A+NTSS+P+ Sbjct: 365 VEETARRLRADVEGKGVAAADLAIEAIFENAEAKQALYASIEPQFQADEILATNTSSIPL 424 Query: 430 GDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRD 489 ++ P++ +GLHFF+PV +MPLVE++ H G + + K GK P+ V+ Sbjct: 425 DELRRGLKAPQRFLGLHFFNPVAQMPLVEVVRHDGLDPEVEKRALAFCKAIGKLPVAVKG 484 Query: 490 KAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIP 549 GF VNRIL PY+ EAIR+ +G +D KFG P+GPI+L D VG+D + Sbjct: 485 TPGFLVNRILMPYLLEAIRLYNEGVPGPVLDKEAKKFGMPMGPIELADTVGLDVCASVGK 544 Query: 550 VLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQG 609 L G P + L ++G+K+G+GFY++ Q+G+ K +VDP Sbjct: 545 ELAPFLG--LELPPGIEDK-LAAGKRGKKDGQGFYVW-QEGKPQKPEVDP---------- 590 Query: 610 QGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSL 669 + ER ++ M+NEAV C+ + V+ D G +FG GF PF GGP +Y+ S Sbjct: 591 -DYAVPADLQERMLLPMVNEAVACLADGVVDDADLLDAGVIFGTGFAPFRGGPIQYVRSE 649 Query: 670 GAGEVVAIMQRLATQYGSRFTP 691 GA + ++RLA ++G RFTP Sbjct: 650 GAAVIKGKLERLAQRHGERFTP 671 Lambda K H 0.319 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1035 Number of extensions: 57 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 714 Length of database: 686 Length adjustment: 39 Effective length of query: 675 Effective length of database: 647 Effective search space: 436725 Effective search space used: 436725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory