GapMind for catabolism of small carbon sources

 

Aligments for a candidate for ivdG in Dyella japonica UNC79MFTsu3.2

Align 3-hydroxy-2-methylbutyryl-CoA dehydrogenase subunit (EC 1.1.1.178) (characterized)
to candidate N515DRAFT_3783 N515DRAFT_3783 NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family

Query= metacyc::MONOMER-11802
         (255 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3783 N515DRAFT_3783
           NAD(P)-dependent dehydrogenase, short-chain alcohol
           dehydrogenase family
          Length = 255

 Score =  273 bits (697), Expect = 3e-78
 Identities = 140/255 (54%), Positives = 180/255 (70%)

Query: 1   MHIANKHFIVSGAASGLGAATAQMLVEAGAKVMLVDLNAQAVEAKARELGDNARFAVADI 60
           M +     I++G ASGLG A AQ LV AG +V L D+N    +  AR LG +A F   D+
Sbjct: 1   MQLQQVKAIITGGASGLGYAVAQHLVAAGGQVALFDVNEDKGQEAARALGASAHFFRTDV 60

Query: 61  SDEQAAQSAVDAAVSAFGSLHGLVNCAGIVGAEKVLGKQGPHGLASFAKVINVNLIGSFN 120
           + E    + V AA  A G L+ ++NCAGI+GA +VLGK+GP  L +FA  + VNL+GSFN
Sbjct: 61  TSEDGVSANVAAAREAMGGLNVVMNCAGILGAGRVLGKEGPMPLGTFASTVMVNLVGSFN 120

Query: 121 LLRLAAAAMAEGAADESGERGVIINTASIAAYDGQIGQAAYAASKGAIASLTLPAARELA 180
           + + AAA M    A E GERGVI+NTAS+AAY+GQIGQAAY+ASKG +  +TLP AREL+
Sbjct: 121 VAKAAAALMQSNEAGEDGERGVIVNTASVAAYEGQIGQAAYSASKGGVVGMTLPMARELS 180

Query: 181 RFGIRVMTIAPGIFETPMMAGMSDEVRASLAAGVPFPPRLGRPQEYAALARHIIENSMLN 240
           RFGIRV TIAPGIF TPM+ GM  +V+ SL+A +PFP RLGRP+E+A+L   I+ N  +N
Sbjct: 181 RFGIRVATIAPGIFWTPMVDGMPPQVQESLSASIPFPSRLGRPEEFASLVAFILTNRYIN 240

Query: 241 GEVIRLDGALRMAAK 255
           GE IRLDGA+R+  K
Sbjct: 241 GETIRLDGAVRLQPK 255


Lambda     K      H
   0.318    0.131    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 173
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 255
Length adjustment: 24
Effective length of query: 231
Effective length of database: 231
Effective search space:    53361
Effective search space used:    53361
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory