GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hisP in Dyella japonica UNC79MFTsu3.2

Align Amino-acid ABC transporter, ATP-binding protein (characterized, see rationale)
to candidate N515DRAFT_1821 N515DRAFT_1821 putative ABC transport system ATP-binding protein

Query= uniprot:Q88GX0
         (260 letters)



>FitnessBrowser__Dyella79:N515DRAFT_1821
          Length = 238

 Score =  149 bits (377), Expect = 4e-41
 Identities = 85/222 (38%), Positives = 135/222 (60%), Gaps = 4/222 (1%)

Query: 34  HVLRDIDLQVREGERIVLCGPSGSGKSTLIRCINRLEVAQQGSIQVDGIDLAATTREA-A 92
           + LRD ++ V+EGE + + GPSGSGK+T +     LE    G   +DG++++     A +
Sbjct: 19  YALRDFNIDVKEGEFVAVTGPSGSGKTTFLTIAGLLETFTGGEYHLDGVEVSNLNDNARS 78

Query: 93  QVRSD-IGMVFQHFNLFPHMSVLDNCLLAPTSVRGLSRKDAEERARMYLSKVGIESQAHK 151
           ++R++ IG +FQ FNL P ++V DN +  P   RG+   + ++R    L +VG+ S+A  
Sbjct: 79  KIRNEKIGFIFQAFNLIPDLNVYDN-VEVPLRYRGMKALERKQRIMDALERVGLASRAKH 137

Query: 152 YPSQLSGGQQQRVAIARALCMKPRIMLFDEPTSALDPEMVAEVLDVLVQLAGTGMTMLCV 211
           YP++LSGGQQQRVAIARAL   PR++L DEPT  LD +M   V+++L ++   G T++ V
Sbjct: 138 YPAELSGGQQQRVAIARALAGSPRLLLADEPTGNLDTQMARGVMELLEEIHREGATIVMV 197

Query: 212 THEMGFARQVAERVLFLEGGQIIEDSPPQVFFNQPRTERAKA 253
           TH+   A + A+R + +  GQ+++ +    F  Q     A A
Sbjct: 198 THDPELATR-AQRNVHVIDGQVVDLAEDPRFHQQQARAGAPA 238


Lambda     K      H
   0.322    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 164
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 238
Length adjustment: 24
Effective length of query: 236
Effective length of database: 214
Effective search space:    50504
Effective search space used:    50504
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory