Align mannitol 2-dehydrogenase (NADP+) (EC 1.1.1.138) (characterized)
to candidate N515DRAFT_0039 N515DRAFT_0039 L-threonine 3-dehydrogenase
Query= BRENDA::Q1ACW3 (345 letters) >FitnessBrowser__Dyella79:N515DRAFT_0039 Length = 344 Score = 146 bits (369), Expect = 7e-40 Identities = 100/298 (33%), Positives = 156/298 (52%), Gaps = 16/298 (5%) Query: 3 LPATMKALRYDKPESYA-VVEVPLPTLRDNDVLIKVKACGVCGTDLHIHEGEFIAKFPLI 61 +P TMKAL PE + EVP+P + N+VLIK++ +CGTDLHI++ + ++ + Sbjct: 1 MPQTMKALVKRLPEQGIWMEEVPVPEVGPNEVLIKMEKTAICGTDLHIYKWDEWSQRTIK 60 Query: 62 PG----HETVGVVAAIGKDVKGFTVGERVCADNSELCNECFYCRRGQLLLCEKFEAHGVT 117 PG HE VG + IG V G+ VG+RV A+ +C C CR G+ LC GV Sbjct: 61 PGLTIGHEFVGRIVDIGPGVTGYKVGDRVSAEGHIVCGHCRNCRAGRQHLCPNTVGIGVN 120 Query: 118 MDGGFAEYCAYPAGKVFKI-HNLSDVDATLLEPASCAAH-GLEKIAPKIGSSVLMFGAGP 175 +G FAEY PA ++ I + A +P AAH LE IG VL+ GAGP Sbjct: 121 RNGAFAEYMTMPASNLWPIPDQIPSELAAFFDPYGNAAHCALE--FDLIGEDVLITGAGP 178 Query: 176 TGLCLAQL-PHNGASHVVIAAPEGLKMDLAKKLDCADIYVPLSRSNPQAQFDQIKSDNPY 234 G+ A + H GA +VV+ ++ LA + + ++ +N Q+ D +K + Sbjct: 179 IGIIAAGIAKHVGARNVVVTDVNDYRLKLAADMGATRV---VNVAN-QSLRDVVKDLHIE 234 Query: 235 GFDIVVEATGSPKILEDAINYVRRGGKLVVYGVYSDAARVSWPPSKIFGDEITIIGSF 292 GFD+ +E +G+P+ D ++ + GGK+ + G+ A + W K+ +T+ G + Sbjct: 235 GFDVGLEMSGNPRAFNDMLDCMYHGGKIALLGIMPRGAGIDW--DKVIFKGLTLQGIY 290 Lambda K H 0.320 0.138 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 346 Number of extensions: 25 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 345 Length of database: 344 Length adjustment: 29 Effective length of query: 316 Effective length of database: 315 Effective search space: 99540 Effective search space used: 99540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory