Alignments for a candidate for ARO10 in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Alignments for a candidate for ARO10 in Dyella japonica UNC79MFTsu3.2

Align phenylpyruvate decarboxylase (EC 4.1.1.43) (characterized)
to candidate N515DRAFT_1617 N515DRAFT_1617 indolepyruvate decarboxylase

Query= BRENDA::Q2UKV4
         (570 letters)



>FitnessBrowser__Dyella79:N515DRAFT_1617
          Length = 590

 Score =  200 bits (509), Expect = 1e-55
 Identities = 169/581 (29%), Positives = 272/581 (46%), Gaps = 63/581 (10%)

Query: 16  VAEYLFRRLREVGVRAVHGVPGDYN---LVALDYLPKCDLHWVGNCNELNAGYAADGYAR 72
           VA+YL  RL+++ V  V  +PGDY      AL+Y     ++ +G  NEL+A YAAD YAR
Sbjct: 27  VADYLLTRLKQLNVTDVFQIPGDYVKHFTQALEYFD--GVNTIGAINELDAAYAADAYAR 84

Query: 73  INGMSALVTTFGVGELSALNAIAGAYSEFVPIVHIVGQPHTK----SQKDGMLLHHTLGN 128
             G++A+   FGV   SALNAIAGA+ E  PIV I   P       +    +L HH+   
Sbjct: 85  TRGLAAVSLQFGVSTYSALNAIAGAWVERSPIVVISATPGADMRDITDMYDVLFHHS--T 142

Query: 129 GDFNVFTRMSADISCTLGCLNSTHEVATLIDNAIRECWIRSRPVYISLPTDMVTKKIEGE 188
           GD N    +   ++     L++    A  ID  + +     RPVYI+       K++ G+
Sbjct: 143 GDLNSDRAIYEYVTVKAITLSTNVGAAEQIDELLVDAITEKRPVYIA-----CYKEVWGQ 197

Query: 189 RLDTPLDLSLPPNDPEKEDY----VVDVVLKYLHAAKKPVILVDACAIRHRVLDEVHEFV 244
             + P    L P      +      VD     +  AK P+I      +R  +   + + +
Sbjct: 198 PCERPPATPLKPRVTRSPELALRNAVDQAWAMITQAKSPLIFAGVEVLRFGLSGLLQKII 257

Query: 245 EKSGLPTFVAPMGKGAVDETHKNYGGVYAGTGSNPGVREQVESSDLILSIGAIKSDFNTT 304
           + SG       +GK  +DE    + G Y+   S   VRE V +SD +L++GAI +D   T
Sbjct: 258 DASGFLYTTTTLGKSVLDEQGDKFIGTYSDAASIEAVREIVMASDCVLTLGAIITDDYLT 317

Query: 305 GFSYRIGQLNTIDFHSTYVRVRYSEYPDINMKGVLQKIVQRMGNLNVGPV---SPPSNLL 361
               +   +   D  +  VR  Y +Y D+ M+  ++ ++ R       P+   +PP    
Sbjct: 318 FIEAKYADMVLAD--TAGVRAGYFKYGDVTMRDFMEALLARFTASKAYPIKAKAPPQPKY 375

Query: 362 PDNEKASTE-------QAITHAWLWPTVGQWLKEKDVVITETGTANFGIWDTRFPA---- 410
           P    A+++       Q IT+   +    ++L++KD++  +     +G+  + + A    
Sbjct: 376 PQPWAANSDPVYDAQPQIITYNRFFAHTMKFLQDKDLL--KDIVMTYGVSSSMYVATNCY 433

Query: 411 GV---TAISQVLWGSIGYSVGACQGAALAAKEQGRRTVLFVGDGSFQLTLQEVSTMIRNN 467
           G+   + +S   W  IG+  GA  GA L +   G+R     GDG F +  Q +ST+ RN 
Sbjct: 434 GLKQGSFVSSAAWQCIGFETGAACGAQLGS---GKRAWTVAGDGGFMMVCQSLSTLARNQ 490

Query: 468 LNPIIFVICNEGYTIER-YI----------HGWEAVYNDIQPWDFLNIPVAFGAKDKYKG 516
           LN +IFV+ N+ Y IE+ Y+          H ++A ++ +  WD+L +  AFGA    +G
Sbjct: 491 LNAVIFVMSNQVYAIEQVYVDMSAFKPGPKHKFDA-FDILPKWDYLALAKAFGA----EG 545

Query: 517 YKVTTRDELRELFAN-EEFASAPCLQLVELHMPRDDCPASL 556
            +V T DEL        +    P   LVE+ +P+ D P  +
Sbjct: 546 IRVETVDELNAALPRIAKIKDKPV--LVEVVIPQKDLPGQM 584


Lambda     K      H
   0.319    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 698
Number of extensions: 40
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 570
Length of database: 590
Length adjustment: 36
Effective length of query: 534
Effective length of database: 554
Effective search space:   295836
Effective search space used:   295836
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources