GapMind for catabolism of small carbon sources

 

Aligments for a candidate for livF in Dyella japonica UNC79MFTsu3.2

Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate N515DRAFT_4159 N515DRAFT_4159 ABC-2 type transport system ATP-binding protein

Query= uniprot:A0A165KC78
         (242 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_4159 N515DRAFT_4159 ABC-2
           type transport system ATP-binding protein
          Length = 245

 Score =  102 bits (253), Expect = 9e-27
 Identities = 68/215 (31%), Positives = 118/215 (54%), Gaps = 12/215 (5%)

Query: 9   LLQVKGLKVAYGGIQAVKGVDFEVREGELVSLIGSNGAGKTTTMKAITGTLSMNDGNIEY 68
           LL++  ++    G  AV+ +   +  G+++ L+G NGAGK+TT+  I G L  ++G IE 
Sbjct: 7   LLKLDQVRRHRAGRPAVERLSLTLDRGQILGLLGVNGAGKSTTLAMIAGALKPDEGRIEL 66

Query: 69  LGKSIKGKGAWDLVKEGLVMVPEGRGVFARMTITENLQMGAYIRKDKAGIL-----ADIE 123
            G+      A  L +  L  +PE    +  +T+ E+LQ  A +R  +AG L     + IE
Sbjct: 67  DGQDFATHPA--LARGLLGWLPERAPHWPELTVLEHLQAHARLRGLRAGGLKQACESTIE 124

Query: 124 KMFTIFPRLRERKDQLAGTMSGGEQQMLAMGRALMSQPKVLLLDEPSMGLSPIMVDKIFE 183
           ++  + P LR    +LAG +S G++Q L +  AL+ +P++L+LDEP+  L P+ V  + +
Sbjct: 125 RL-ELAPLLR----RLAGVLSQGQRQRLGLACALLHKPRLLVLDEPANALDPVQVAALRK 179

Query: 184 VVRDVYALGVTIVLVEQNASRALAIADRGYVMESG 218
           V+R+  A G  ++L     +   A+ DR  ++  G
Sbjct: 180 VIREEAAAGAAVILSTHLLAETTAVCDRVAILHEG 214


Lambda     K      H
   0.317    0.136    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 111
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 242
Length of database: 245
Length adjustment: 24
Effective length of query: 218
Effective length of database: 221
Effective search space:    48178
Effective search space used:    48178
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory