GapMind for catabolism of small carbon sources

 

Aligments for a candidate for proP in Dyella japonica UNC79MFTsu3.2

Align proline porter II (characterized)
to candidate N515DRAFT_3073 N515DRAFT_3073 Arabinose efflux permease

Query= CharProtDB::CH_024324
         (500 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3073 N515DRAFT_3073
           Arabinose efflux permease
          Length = 551

 Score =  210 bits (534), Expect = 1e-58
 Identities = 122/325 (37%), Positives = 180/325 (55%), Gaps = 16/325 (4%)

Query: 24  RKAITAASLGNAMEWFDFGVYGFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGG 83
           R+ I A+SLG   EW+DF +YG +A  +GK FF G + +   + AL  F+  F +RP G 
Sbjct: 16  RRVILASSLGTVFEWYDFYLYGSLAVLIGKHFFSGLNETSAFIFALLAFAAGFAVRPFGA 75

Query: 84  LFFGMLGDKYGRQKILAITIVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGG 143
           + FG LGD  GR+    ITI+IM +STF +G++PSY T+G+ AP+LL++ ++ QG ++GG
Sbjct: 76  IVFGRLGDLVGRKYTFLITIIIMGLSTFLVGVLPSYSTVGMIAPVLLIVLRLLQGLALGG 135

Query: 144 EYTGASIFVAEYSPDRKRGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIP 203
           EY GA+ +VAE++PD KRG   S++   +  G  L   V++     +G+  F DWGWRIP
Sbjct: 136 EYGGAATYVAEHAPDGKRGLYTSFIQITATFGLFLSLLVILGTRMALGDKVFEDWGWRIP 195

Query: 204 FFIALPLGIIGLYLRHALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSL-- 261
           F I+  L  + +Y+R  L+E+P FQQ          EG     K    E A  +W++L  
Sbjct: 196 FLISSVLVAVSVYIRLQLQESPVFQQM-------KAEGKHS--KAPLTE-AFGHWKNLKV 245

Query: 262 --LTCIGLVIATNVTYYMLLTYMPSYLSHNLHY-SEDHGVLIIIAIMIGMLFVQPVMGLL 318
             L  +G      V +Y    Y   +L+ +L        +LI  A++IG  F     G L
Sbjct: 246 VILALLGATAGQAVVWYTGQFYALYFLTQSLKIDGTTANLLIAAALIIGTPFF-VFFGWL 304

Query: 319 SDRFGRRPFVLLGSVALFVLAIPAF 343
           SDR GR+  VL G +   +   P F
Sbjct: 305 SDRIGRKSIVLAGCLLAALTYFPIF 329



 Score = 39.7 bits (91), Expect = 3e-07
 Identities = 26/86 (30%), Positives = 46/86 (53%), Gaps = 2/86 (2%)

Query: 358 LLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAAFNI-SVLVAGLTPTLAAWLVESSQN 416
           L +L + +    G +A+ L  MFPT IRY++++  ++I +    G  PT+   LV    +
Sbjct: 459 LAILVLYVTMVYGPIAAWLVEMFPTRIRYTSMSLPYHIGNGWFGGFVPTIGFMLVAWKGD 518

Query: 417 LMMPAYYLMVVAVVGLITGVT-MKET 441
           +    +Y ++VAV     G+  +KET
Sbjct: 519 IYYGLWYPIIVAVGTFFIGLLFLKET 544


Lambda     K      H
   0.327    0.142    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 711
Number of extensions: 45
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 500
Length of database: 551
Length adjustment: 35
Effective length of query: 465
Effective length of database: 516
Effective search space:   239940
Effective search space used:   239940
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory