Align Acyl-coenzyme A synthetase ACSM3, mitochondrial; Acyl-CoA synthetase medium-chain family member 3; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; Middle-chain acyl-CoA synthetase 3; Propionate--CoA ligase; Protein SA homolog; EC 6.2.1.2; EC 6.2.1.17 (characterized)
to candidate N515DRAFT_1417 N515DRAFT_1417 long-chain acyl-CoA synthetase
Query= SwissProt::Q3UNX5 (580 letters) >FitnessBrowser__Dyella79:N515DRAFT_1417 Length = 562 Score = 176 bits (445), Expect = 3e-48 Identities = 155/544 (28%), Positives = 245/544 (45%), Gaps = 54/544 (9%) Query: 66 EKAGKRLSNPAFWWIDGNGEELRWSFEELGLLSRKFANILTEACSLQRGDRVMVILPKIP 125 E K PAF G+EL S+ ++ LSR+FA LT L +GDR+ +++P + Sbjct: 33 EAFAKFRDRPAF---HSFGKEL--SYGQIDELSRQFAGYLTGVLKLGKGDRIAIMMPNVL 87 Query: 126 EWWLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKAKCIITDDTLAPAVDAVAAKCENL 185 ++ +A LR G V++ T +++ ++L+ + AK I+ D A + V A+ E Sbjct: 88 QYPIALFGALRAGLVVVNTNPMYTARELKHQLEDAGAKAIVVLDNFAATLQQVVAETEVE 147 Query: 186 HSKLIVSQHSREGWGN--------LKEMMKYASD-----------------SHTC--VDT 218 H IV+ + G LK + K D +H VD Sbjct: 148 H---IVTTGIGDLLGLKGTLINFVLKHIKKMVPDYRLPHAVRFRDALAQGAAHPVPKVDL 204 Query: 219 KHDEMMAIYFTSGTTGPPKMIGHTHSSFGLGLSVNGRFWLDLIAS---DVMWNTSDTGWA 275 HD++ + +T GTTG K +H + + G W+ A +V+ Sbjct: 205 GHDDLAFLQYTGGTTGVAKGAMLSHGNMVANMLQAGA-WIGTNAKPGEEVIITALPLYHI 263 Query: 276 KSAWSSVFSPWTQGACVFAHYLPRFESTSILQTLSKFPITVFCSAPTAYRMLVQND-MSS 334 S ++ G + PR + ++ L K T T + L+ + Sbjct: 264 FSLTANGLVFTRLGGLNWLITNPR-DMPGFVKELKKSRFTALTGVNTLFNGLLNTPGFAE 322 Query: 335 YKFNSLKHCVSAGEPINPEVMEQWRKKTGLDIYEGYGQTETV-LICGNFKGMKIKPGSMG 393 F+ L + G + V E+W+K TG+ + E YG TET C N +K S+G Sbjct: 323 VDFSRLHLSLGGGMAVQRAVAERWKKVTGVTLAEAYGLTETSPAACINPLDLKDYNSSIG 382 Query: 394 KPSPAFDVKILDENGATLPPGQEGDIALQVLPERPFGLFTHYVDNPSKTASTLRGSFYI- 452 P P+ DV I E+ LP G+ G++ ++ P+ G Y P +TA L ++ Sbjct: 383 LPIPSTDVAIWSEDSQPLPIGEVGELMVKG-PQVMQG----YWKRPDETAKVLGADGWLH 437 Query: 453 TGDRGYMDEDGYFWFVARSDDIILSSGYRIGPFEVESALIEHPSIAESAVVSSPDPIRGE 512 TGD MD +GY + V R D+IL SG+ + P EVE ++ HP +AE A V PD GE Sbjct: 438 TGDIARMDANGYVYIVDRKKDMILVSGFNVYPNEVEDIVMTHPGVAEVAAVGVPDEHSGE 497 Query: 513 VVKAFIVLNPDYKSHDQEQLKKEIQEHVKKTTAPYKYPRKVEFIEELPKTVSGKVKRNEL 572 VVK F+V D + ++E ++ YK P+ +EF + LPK+ GK+ R EL Sbjct: 498 VVKLFVV------RKDPNLTVEALKEFCRENLTGYKRPKLIEFRDALPKSNVGKILRREL 551 Query: 573 RKKE 576 R ++ Sbjct: 552 RDEK 555 Lambda K H 0.319 0.134 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 759 Number of extensions: 35 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 3 Length of query: 580 Length of database: 562 Length adjustment: 36 Effective length of query: 544 Effective length of database: 526 Effective search space: 286144 Effective search space used: 286144 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory