Alignments for a candidate for cstA in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Alignments for a candidate for cstA in Dyella japonica UNC79MFTsu3.2

Align Carbon starvation protein A (characterized, see rationale)
to candidate N515DRAFT_4411 N515DRAFT_4411 carbon starvation protein

Query= uniprot:A0A0N9VZ52
         (685 letters)



>FitnessBrowser__Dyella79:N515DRAFT_4411
          Length = 691

 Score =  902 bits (2332), Expect = 0.0
 Identities = 422/681 (61%), Positives = 547/681 (80%), Gaps = 3/681 (0%)

Query: 5   AKHLAWFAVAVLGAFALSVVALRRGEAINALWIVVAAVAIYLVAYRYYSLFIANKVMQLD 64
           A  + W A+A++GA+ L VVALRRGE INA+W+V AA+A++++ YR+YS FI   V+++D
Sbjct: 11  AGKILWAAIAIVGAWCLGVVALRRGEPINAIWLVAAAIAVFVIGYRFYSRFIDTYVLRMD 70

Query: 65  PGRATPAVVNNDGLDYVPTNKHILFGHHFAAIAGAGPLVGPVLAAQMGYLPGTLWLIAGV 124
           P RATPAV+ NDGLDYVPT+K ++FGHHFAAIAGAGPLVGPVLAAQMGYLPGTLW++ GV
Sbjct: 71  PSRATPAVLRNDGLDYVPTDKWVVFGHHFAAIAGAGPLVGPVLAAQMGYLPGTLWILFGV 130

Query: 125 VLAGAVQDFMVLFMSTRRNGRSLGDMVREEMGRVPGTIALFGCFLIMIIILAVLALIVVK 184
           V AGAVQDFM+L +S RR+GRSLG+M+R+E+G V G +A+FG  ++M+I+LAVLAL+VVK
Sbjct: 131 VFAGAVQDFMILGLSVRRDGRSLGNMLRDELGPVAGIVAMFGVLVLMMIVLAVLALVVVK 190

Query: 185 ALADSPWGMFTVMATIPIAMFMGVYMRYIRPGRIGEISVVGVLLLLGSIWLGGQIAADPV 244
           AL  SPWG +TV  TIPIA+ MG+Y+RYIRPG+I E+S+VG++LLL +IW G  +AA P 
Sbjct: 191 ALTHSPWGTYTVACTIPIALLMGIYLRYIRPGKILEVSIVGLILLLLAIWSGAYVAASPA 250

Query: 245 WAKAFSFTGIQITWMLIGYGFVAAVMPVWLILAPRDYLSTFLKIGTIMALAIGILITMPE 304
           WA AF F    + W+LI YGF A+V+PVWL+LAPRDYLSTFLKIGTI  LA+ I +  P 
Sbjct: 251 WAAAFDFDAKALAWLLIAYGFCASVLPVWLLLAPRDYLSTFLKIGTIALLALAIFLAAPT 310

Query: 305 LKMPALTQFIDGTGPVWKGGLFPFLFITIACGAVSGFHALIASGTTPKLLASEGHARYIG 364
           L+MPALT+F+DGTGPV++G LFPFLFITIACGAVSG+H++I+SGTTPKLL++EG AR +G
Sbjct: 311 LQMPALTKFVDGTGPVFQGNLFPFLFITIACGAVSGWHSIISSGTTPKLLSNEGEARMVG 370

Query: 365 YGGMLMESFVAIMAMVAASVIEPGVYFAMNSPAAIVGGDVVQVAQTVSSWGFMITPEALQ 424
           YGGMLME+FVAIMA++AA+ + PGVYFAMNSPAA++G      A  +S WGF+++P+ L 
Sbjct: 371 YGGMLMEAFVAIMALIAAASMHPGVYFAMNSPAALIGTTAQSAATAISQWGFVVSPDELL 430

Query: 425 AVAKDIGETTVLARAGGAPTLAVGIAQILHSVLPGENTMAFWYHFAILFEALFILTAVDA 484
             A++IGE+++L+RAGGAPTLAVG+AQ+LH ++PGE  MAFWYH+AILFEALFILT VDA
Sbjct: 431 NTARNIGESSILSRAGGAPTLAVGMAQLLHGIIPGEGMMAFWYHYAILFEALFILTTVDA 490

Query: 485 GTRAGRFMLQDLLGSFVPALKRTESWTANLIATAGCVAMWGYLLYQGVIDPLGGINTLWP 544
           GTR GRFM+Q++ G     LK TESWT NLIATA CV +WGY LYQG +DPLGGINTLWP
Sbjct: 491 GTRVGRFMIQEIAGLIHEPLKHTESWTGNLIATAICVGLWGYFLYQGAVDPLGGINTLWP 550

Query: 545 LFGISNQMLAGIALMLSTVVLIKMKRQRYIWVTLLPAAWLLICTTTAGFIKLFDANPAIG 604
           LFGI+NQMLA +ALML+TVV +K+KR+RY+WV  +PA WL++CT TAG+ KL     +I 
Sbjct: 551 LFGIANQMLAAVALMLATVVTVKLKRERYVWVPGIPAIWLVVCTLTAGYYKLVG---SIS 607

Query: 605 FLSLAKKYSDALANGQILAPAKDITQMQHVIYNAYTNATLTALFLFVVFSILFYAIKVGI 664
           F + A+KY+ A A G++LAPAK + +MQ ++ N Y +A LT +F+ +V ++  +AI   +
Sbjct: 608 FTAAAQKYAAAAAEGKLLAPAKSVAEMQRIVTNNYVDAALTGIFMLLVVTMAGFAINAIL 667

Query: 665 SAWGSKERTDKESPFQAIPEA 685
            AW     T  E P+ A+  A
Sbjct: 668 KAWRVNHPTAHEEPYVALEGA 688


Lambda     K      H
   0.328    0.141    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1394
Number of extensions: 52
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 685
Length of database: 691
Length adjustment: 39
Effective length of query: 646
Effective length of database: 652
Effective search space:   421192
Effective search space used:   421192
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources