GapMind for catabolism of small carbon sources

 

Aligments for a candidate for ltaE in Dyella japonica UNC79MFTsu3.2

Align Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1; L-threonine/L-allo-threonine aldolase; EC 4.1.2.48 (uncharacterized)
to candidate N515DRAFT_1841 N515DRAFT_1841 glycine hydroxymethyltransferase

Query= curated2:D3DKC4
         (427 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_1841 N515DRAFT_1841 glycine
           hydroxymethyltransferase
          Length = 417

 Score =  514 bits (1323), Expect = e-150
 Identities = 257/406 (63%), Positives = 315/406 (77%), Gaps = 4/406 (0%)

Query: 8   DAEIYEAIVKEYERQFYHLELIASENFTSLAVMEAQGSVMTNKYAEGLPHKRYYGGCEFV 67
           D E+ +A+  E  RQ  H+ELIASEN+ S  V+EAQGSV+TNKYAEG P KRYYGGCE+V
Sbjct: 12  DDELAQAMADEARRQEDHVELIASENYASPRVLEAQGSVLTNKYAEGYPGKRYYGGCEYV 71

Query: 68  DIAEDLAIERAKALFDAEHANVQPHSGTQANMAVYMAVLKPGDTIMGMDLSHGGHLTHGA 127
           D+AE LAIER K LF A +ANVQPHSG+QAN AVY A+L PGDTI+GM L+HGGHLTHGA
Sbjct: 72  DVAERLAIERLKELFGATYANVQPHSGSQANQAVYFALLNPGDTILGMSLAHGGHLTHGA 131

Query: 128 KVNFSGKIYNAVYYGVHPETHLIDYDQLYRLAKEHKPKLIVGGASAYPRVIDWAKLREIA 187
           KVN SGKI NAV YGV+ E  L+DYD++ R+A EHKPK+IV G SAY +V+DWA+ R IA
Sbjct: 132 KVNLSGKILNAVQYGVN-EQGLVDYDEVERMALEHKPKMIVAGFSAYSQVMDWARFRAIA 190

Query: 188 DSVGAYLMVDMAHYAGLIAGGVYPNPVPYAHFVTSTTHKTLRGPRSGFILCK---KEFAK 244
           D VGAYL VDMAH AGLIA GVYP+P+P+AH VTSTTHKTLRGPR G I+ K   +E  K
Sbjct: 191 DKVGAYLFVDMAHVAGLIAAGVYPSPLPHAHVVTSTTHKTLRGPRGGIIVAKDAGEEIEK 250

Query: 245 DIDKSVFPGIQGGPLMHVIAAKAVAFKEAMSQEFKEYARQVVANARVLAEEFIKEGFKVV 304
            +   VFPGIQGGPLMHVIAAKAVAFKEA+   FK Y +QVV NA+ +A+ FI+ G+K+V
Sbjct: 251 KLQSIVFPGIQGGPLMHVIAAKAVAFKEALDPSFKSYQQQVVKNAKAMAKTFIERGYKIV 310

Query: 305 SGGTDSHIVLLDLRDTGLTGREVEEALGKANITVNKNAVPFDPLPPVKTSGIRLGTPAMT 364
           SGGT++H++L+DL    +TG++ EEALGKA+ITVNKNAVP DP  P  TSG+R+GTPA+T
Sbjct: 311 SGGTENHLMLVDLIGREVTGKDAEEALGKAHITVNKNAVPNDPRKPFVTSGLRIGTPAVT 370

Query: 365 TRGMKEDQMRIIARLISKVIKNIGDEKVIEYVRQEVIEMCEQFPLY 410
           TRG +E  +  +A  I  V+    DE V+  VR++V   C +FP+Y
Sbjct: 371 TRGYQEADVVALAGWICDVLDAPNDEAVLAKVREQVTAQCHKFPVY 416


Lambda     K      H
   0.319    0.136    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 616
Number of extensions: 25
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 427
Length of database: 417
Length adjustment: 32
Effective length of query: 395
Effective length of database: 385
Effective search space:   152075
Effective search space used:   152075
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory