GapMind for catabolism of small carbon sources

 

Aligments for a candidate for serP1 in Dyella japonica UNC79MFTsu3.2

Align Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized)
to candidate N515DRAFT_3653 N515DRAFT_3653 amino acid/polyamine/organocation transporter, APC superfamily (TC 2.A.3)

Query= TCDB::F2HQ25
         (459 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3653 N515DRAFT_3653 amino
           acid/polyamine/organocation transporter, APC superfamily
           (TC 2.A.3)
          Length = 453

 Score =  267 bits (683), Expect = 5e-76
 Identities = 158/444 (35%), Positives = 240/444 (54%), Gaps = 17/444 (3%)

Query: 11  QRGLQNRHIQLIAIAGTIGTGLFLGAGKTIQMTGPSVIFAYILIGIAMFFFLRTIGEMLY 70
           QR L  RHI  +A+   IG GLFLG+   I + GPSV+FAY+  G  +F  +R +GEM  
Sbjct: 6   QRRLTPRHITFMALGMAIGAGLFLGSANAINLAGPSVLFAYLFGGAMIFIIMRALGEMAV 65

Query: 71  NDPSQHSFLNFVTKYSGVRTGYFTQWSYWLVIVFVCISELTAIGTYIQFWLPQVPLWLIE 130
           +DP   SF  +  +Y G   GY T W+YW+++V V ++E TA+G Y++ W P++P W+  
Sbjct: 66  HDPVAGSFSTYAHRYLGPFAGYLTGWNYWILMVGVGMAESTAVGIYMRQWFPELPQWIWV 125

Query: 131 IVMLALLFGLNTLNSRFFGETEFWFAMIKVAAIIGMIVTAIILVAGNFHYSTVLSGKTVH 190
              +A++ GLN +  + +GE EFWF +IKV  ++ MI+      AG         G+ V 
Sbjct: 126 FGSVAMIGGLNLMAVKVYGEMEFWFTLIKVVTVVLMILGG----AGMIWLGWGNGGQPV- 180

Query: 191 DSASLSNIFDGFQLFPHGAWNFVGALQMVMFAFTSMEFIGMTAAETVNPKKSLPKAINQI 250
               L+N++     FPHG    V AL +V+FAF  +E IGM A E   P++++P+A+N +
Sbjct: 181 ---GLANLWSHGGWFPHGFTGMVLALPVVVFAFGGIETIGMAAGEAAQPERTIPRAVNSV 237

Query: 251 PVRILLFYVGALLAIMAIFNWHYIPADKSPFVMVFQLIGIKWAAALINFVVLTSAASALN 310
             RIL+FYVGAL  IMAI+ W  +    SPFV  F  +GI  AA LINFVV+T+A S  N
Sbjct: 238 LWRILIFYVGALFVIMAIYPWDQLGTQGSPFVTTFGKLGIPQAAGLINFVVITAALSGFN 297

Query: 311 SSLFSATRNMYSLAQQHDKGRLTPFT-KLSKAGIPINALYMATALSLLAPVLT-LIPQIK 368
           S+ FS +R +YSL+    K +   F  ++S+ G+P+ A+ +  A  +   VL  L+P+  
Sbjct: 298 STTFSGSRMLYSLS---TKAQAPAFLGQVSEHGVPVRAVLVTLACLVFGVVLNYLLPE-- 352

Query: 369 NAFDFAASCTTNLFLVVYFITLYTYWQYRKSEDYNPKGFLTPKPQITVPFIVAIFAIVFA 428
             F    S      +  + + L  ++ +R+   +    F      +T    +   A V  
Sbjct: 353 RIFAMMMSILAFNTVWTWMMVLIAHYSFRRR--HGATAFPLRAWPLTSVVCLLFLAFVLF 410

Query: 429 SLFFNADTFYPALGAIVWTIFFGL 452
            L ++ADT         W +   L
Sbjct: 411 MLGYSADTRVALYVGAGWVVLLSL 434


Lambda     K      H
   0.329    0.141    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 632
Number of extensions: 41
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 459
Length of database: 453
Length adjustment: 33
Effective length of query: 426
Effective length of database: 420
Effective search space:   178920
Effective search space used:   178920
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory