GapMind for catabolism of small carbon sources

 

Alignments for a candidate for eda in Dyella japonica UNC79MFTsu3.2

Align 2-dehydro-3-deoxy-phosphogluconate aldolase (EC 4.1.2.14) (characterized)
to candidate N515DRAFT_3178 N515DRAFT_3178 2-dehydro-3-deoxyphosphogluconate aldolase / (4S)-4-hydroxy-2-oxoglutarate aldolase

Query= BRENDA::Q0K1X1
         (214 letters)



>FitnessBrowser__Dyella79:N515DRAFT_3178
          Length = 216

 Score =  217 bits (552), Expect = 1e-61
 Identities = 108/196 (55%), Positives = 136/196 (69%)

Query: 16  PVIPVLEFHSVDEALHVSEALVTGGLPLLEITLRTPVALEAIKAVAAALPQACVGAGTVL 75
           PV+PV+       A+ ++ ALV GG+P +E+TLRTP AL+AI+A+AA +  A VG GTVL
Sbjct: 19  PVVPVVIIDDARAAVPMARALVAGGIPAIEVTLRTPAALDAIRAIAAEVEGAVVGVGTVL 78

Query: 76  NVEQLHAVRDAGAQFAVSPGLTPALAEGAQGAGISLLPGVATASEAMAALEAGFTFLKFF 135
               L   R AGA+FAVSPG++P L   A  + + LLPGVATASEAM+ LE G+  LKFF
Sbjct: 79  TPRDLEHARQAGAKFAVSPGVSPKLLAAADDSDLPLLPGVATASEAMSLLERGYRHLKFF 138

Query: 136 PAQAAGGVPMLKSLGGPLPQLRFCPTGGIDAALAPTYLALPNVVCVGGSWVVPKDAVASG 195
           PA  AGG  +L +   PLPQ+RFCPTGGI    AP +L+LPNVVCVGGSW+ P D + SG
Sbjct: 139 PAVPAGGHKLLGAWASPLPQIRFCPTGGISLTSAPEFLSLPNVVCVGGSWLTPVDKLKSG 198

Query: 196 DWGRIRTLAEQARALR 211
           DW  I  LA +A AL+
Sbjct: 199 DWAGIEALAREAAALK 214


Lambda     K      H
   0.319    0.135    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 202
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 214
Length of database: 216
Length adjustment: 22
Effective length of query: 192
Effective length of database: 194
Effective search space:    37248
Effective search space used:    37248
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory