Align α,α-trehalase (MSMEG_4535;MSMEG4528) (EC 3.2.1.28) (characterized)
to candidate N515DRAFT_1888 N515DRAFT_1888 Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family
Query= CAZy::ABK72415.1 (668 letters) >FitnessBrowser__Dyella79:N515DRAFT_1888 Length = 620 Score = 252 bits (643), Expect = 4e-71 Identities = 198/624 (31%), Positives = 289/624 (46%), Gaps = 55/624 (8%) Query: 46 GFLSDCETTCLISSAGSVEWLCVPRPDSPSVFGAILD--RGAGHFRLGPYGVSVPAARRY 103 G + + LI + W C+PR DS + F A+L R G + + V A + Y Sbjct: 35 GVIGNGSIAALIDGMARIVWCCLPRFDSDASFCALLSPQREGGDWIIELEHVE-RAEQEY 93 Query: 104 LPGSLILETT-WQTHTGWLIVRDALVMGPWHDIDTRSRTHRRTPMDWDAEHILLRTVRCV 162 LP + +L T + H + + D P H RSR P+ +L R +R + Sbjct: 94 LPNTAVLVTRLYDRHDNAIEITD---FAPRH----RSRGRFYHPV------MLARRIRPI 140 Query: 163 SGTVELVMSCEPAFDYHRVSATWEYSGPAYGEAIARASRNPDSHPTLRLTTNLRIGIEGR 222 G + + P DY A SG + + S RLTT++ + + R Sbjct: 141 QGKPRIRILLRPLCDYG-ARAPETTSGSNHLRFLL-------SDVIQRLTTDVAVPLIER 192 Query: 223 EARARTRLTEGDNVFVALSWSKH----PAPQTYEEAADKMWKTSEA----WRQWINVGDF 274 +F L H P AD + + E WR W+ Sbjct: 193 ------------GLFFHLDRPAHLVFGPDETLVTSPADFIHSSLEYTIAYWRDWVRYLSI 240 Query: 275 PDHPWRAYLQRSALTLKGLTYSPTGALLAAPTTSLPETPQGERNWDYRYSWIRDSTFALW 334 P + W+ + R+A+TLK Y TGA++AA TTS+PE P RNWDYRY W+RD+ F + Sbjct: 241 P-YEWQEPVIRAAITLKLCQYEATGAIIAALTTSIPEAPGTRRNWDYRYCWLRDAAFVVR 299 Query: 335 GLYTLGLDREADDFFSFIADVSGANNGERHPLQVMYGVGGERSLVEEELHHLSGYDNSRP 394 L LG R +++ ++ ++ A H + ++G+ ER L E E L GY P Sbjct: 300 ALNRLGATRSMEEYIRYVFNIFSAEGD--HEVGPVFGITFERELPEREAAGLDGYRGMGP 357 Query: 395 VRIGNGAYNQRQHDIWGT-MLDSVYLHAKSR-EQIPDA-LWPVLKNQVEEAIKHWKEPDR 451 VRIGN A+ QRQ+D++G+ +L S L R E DA + L++ A++ +EPD Sbjct: 358 VRIGNDAWRQRQNDVYGSVVLASAQLFFDQRLENRGDARTFQRLESMGRMALRMAEEPDA 417 Query: 452 GIWEVRGEPQHFTSSKIMCWVALDRGSKLAELQGEKSYAQQWRAIAEEIKADVLARGVDK 511 G+WE RG T S MCW A DR + +A G K A+ W A + + R ++ Sbjct: 418 GLWEFRGRAAVHTYSAAMCWAACDRLAHIATELGLKDRARYWHDEAVALHERISKRIWNE 477 Query: 512 R-GVLTQRYGDDALDASLLLAVLTRFLPADDPRIRATVLAIADELTEDGLVLRYRVEETD 570 R G Y + LDASLLL F+ DPR ATV AI L + RY Sbjct: 478 RLGHFVDAYDGEHLDASLLLLADIGFVRGRDPRFIATVDAIGKALGRGDYLFRY---IAP 534 Query: 571 DGLAGEEGTFTICSFWLVSALVEIGEISRAKHLCERLLSFASPLHLYAEEIEPRTGRHLG 630 D E +F IC+FW + AL G RA+ + E +L+ +PL L +E+I P TG H G Sbjct: 535 DDFGAPETSFNICTFWYIEALAATGRKRRARLMFEHMLAQRNPLGLLSEDIAPGTGEHWG 594 Query: 631 NFPQAFTHLALINAVVHVIRAEEE 654 NFPQ ++ + LI + + R E+ Sbjct: 595 NFPQTYSLVGLIQTAMRLSRRWED 618 Lambda K H 0.319 0.135 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1153 Number of extensions: 57 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 668 Length of database: 620 Length adjustment: 38 Effective length of query: 630 Effective length of database: 582 Effective search space: 366660 Effective search space used: 366660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory