Align High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate N515DRAFT_1248 N515DRAFT_1248 ABC-2 type transport system ATP-binding protein
Query= TCDB::P21630 (233 letters) >FitnessBrowser__Dyella79:N515DRAFT_1248 Length = 315 Score = 113 bits (283), Expect = 4e-30 Identities = 67/215 (31%), Positives = 120/215 (55%), Gaps = 7/215 (3%) Query: 9 TYYGKIQALHDVSVEVKKGEIVTLIGANGAGKSTLLMTLCGSPQAASGSIRYEGEELVGL 68 TY G QAL + +++++GEI L+G NGAGK+TL+ +CG + ++G++ +G ++ L Sbjct: 20 TYKGGFQALKSIDLDIRRGEIFALLGPNGAGKTTLISIICGIVKPSAGTVSADGHDV--L 77 Query: 69 PSSTIMRKSIAVVPE--GRRVFSRLTVEENLAMGGFFTDKDDYQVQMDKVLELFPRLKER 126 + R I +VP+ F + + G F +DD ++ KVL L E+ Sbjct: 78 RDYRLTRAKIGLVPQELSTDAFETVWAAVRFSRGLFGRARDDRHIE--KVLRDLS-LWEK 134 Query: 127 YEQRAGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLGLAPIIIQQIFEIIEQLRREGVTV 186 + + T+SGG ++ + I +AL +P +L LDEP+ G+ + ++E++ +LR GVTV Sbjct: 135 KDAKIMTLSGGMKRRVLIAKALAHEPSILFLDEPTAGVDVELRHDMWEMVRRLRATGVTV 194 Query: 187 FLVEQNANQALKLADRAYVLENGRIVMHDTGAALL 221 L +A ++ADR V+ G +++ + AL+ Sbjct: 195 ILTTHYIEEAEEMADRVGVITRGELILVEDKRALM 229 Lambda K H 0.318 0.136 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 162 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 315 Length adjustment: 25 Effective length of query: 208 Effective length of database: 290 Effective search space: 60320 Effective search space used: 60320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory