Aligments for a candidate for mmsA in Dyella japonica UNC79MFTsu3.2

GapMind for catabolism of small carbon sources

Aligments for a candidate for mmsA in Dyella japonica UNC79MFTsu3.2

Align Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) (characterized)
to candidate N515DRAFT_3729 N515DRAFT_3729 aminomuconate-semialdehyde/2-hydroxymuconate-6-semialdehyde dehydrogenase

Query= reanno::pseudo1_N1B4:Pf1N1B4_1229
         (505 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3729 N515DRAFT_3729
           aminomuconate-semialdehyde/2-hydroxymuconate-6-
           semialdehyde dehydrogenase
          Length = 483

 Score =  246 bits (628), Expect = 1e-69
 Identities = 166/482 (34%), Positives = 251/482 (52%), Gaps = 15/482 (3%)

Query: 15  LIDGEWVESQTTEWHDIVNPATQQVLAKVPFATAAEVDAAISAAHRAFQTWKLTPIGARM 74
           LIDG     +   W ++  PAT +V A  P ++ A+VDAA++AA  A   W  TP   R 
Sbjct: 9   LIDGRLQAPRQERWLEVFEPATGEVFAHCPESSFADVDAAVAAAVAAAPGWAATPSEQRA 68

Query: 75  RIMLKLQALIREHSKRIAVVLSNEQGKTIADAEG-DIFRGLEVVEH-ACSIGSLQMGEFA 132
           R++ +L  LI       A + S + GK ++ A   DI R +  + + A +I        A
Sbjct: 69  RLLQRLADLIEARLDEFAALESRDSGKPLSLARSLDIPRAVSNLRYFAAAIVPWSSESHA 128

Query: 133 ENVAGGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIACGNTFVLKPSEQDPMSTML 192
             +  G   YTLRQP+GV A I+P+N P  +  W    A+A GN  V KPSE  P +  L
Sbjct: 129 MEL--GAINYTLRQPLGVVACISPWNLPLYLFTWKIAPALAAGNAVVAKPSEITPCTAAL 186

Query: 193 LVELAIEAGIPPGVLNVVHG-GKDVVDALCTHKDIKAVSFVGSTAVGTHVYDLAGKHGKR 251
           L EL+IEAG PPGVLN+V G G +V  AL  H+D+KAVSF GST  G  +   A    K+
Sbjct: 187 LGELSIEAGFPPGVLNIVQGRGPEVGQALVEHRDVKAVSFTGSTRTGAQIAAAAAPRFKK 246

Query: 252 VQSMMGAKNHAVVLPDANREQA-LNALVGAGFGAAGQRCMATS-VVVLVGAAKQWLPDLK 309
           +   +G KN A+V  DA+   A L+ +V +GF   G+ C+  S ++V       +     
Sbjct: 247 LSLELGGKNPAIVFADADLSDANLDTIVRSGFANQGEICLCGSRLLVQRSIYDAFRERYL 306

Query: 310 ALAQKLKVNAGSEPGTDVGPVISKRAKARILDLIESGIKEGAKLELDGRDISVPGYEKGN 369
           A  + L+V    E  TD+G ++S+    ++   I     EG ++   G  +++PG   G 
Sbjct: 307 AKVRALRVGDPREAATDLGALVSREHFDKVTGCIAQARAEGGRVLCGGDALALPGPLAGG 366

Query: 370 -FVGPTLFSGVTPEMQIYTQEIFGPVLVVLEVDTLDQAIALVNANPFGNGTGLFTQSGAA 428
            +V PT+  G+ PE     QEIFGPV+ ++  D   QA+A+ N   +G    L+T   + 
Sbjct: 367 WYVAPTVIEGLGPETATNQQEIFGPVVTLIPFDDEAQALAIANGTGYGLAASLWTTDLSR 426

Query: 429 ARKFQTEIDVGQVGINIPI--PVPVPFFSFTGSRGSKLGDLGPYGKQVVQFYTQTKTVTA 486
           A +F  ++D G V IN  +   +  P   F G++ S +G  G  G + ++F+T+ K +  
Sbjct: 427 AHRFGAQLDFGIVWINCWLLRDLRTP---FGGAKQSGVGREG--GVEALRFFTEPKNICI 481

Query: 487 RW 488
           R+
Sbjct: 482 RY 483


Lambda     K      H
   0.319    0.135    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 566
Number of extensions: 30
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 505
Length of database: 483
Length adjustment: 34
Effective length of query: 471
Effective length of database: 449
Effective search space:   211479
Effective search space used:   211479
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources