GapMind for catabolism of small carbon sources

 

Aligments for a candidate for dopDH in Dyella japonica UNC79MFTsu3.2

Align 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26) (characterized)
to candidate N515DRAFT_3729 N515DRAFT_3729 aminomuconate-semialdehyde/2-hydroxymuconate-6-semialdehyde dehydrogenase

Query= metacyc::MONOMER-20632
         (478 letters)



>lcl|FitnessBrowser__Dyella79:N515DRAFT_3729 N515DRAFT_3729
           aminomuconate-semialdehyde/2-hydroxymuconate-6-
           semialdehyde dehydrogenase
          Length = 483

 Score =  297 bits (761), Expect = 5e-85
 Identities = 189/451 (41%), Positives = 243/451 (53%), Gaps = 9/451 (1%)

Query: 28  TNDVVAKVPMGGQAEVDAAVDAARKAFPAWADASPEVRSDLLDKVGSTIIARSADIGRLL 87
           T +V A  P    A+VDAAV AA  A P WA    E R+ LL ++   I AR  +   L 
Sbjct: 30  TGEVFAHCPESSFADVDAAVAAAVAAAPGWAATPSEQRARLLQRLADLIEARLDEFAALE 89

Query: 88  AREEGKTLAEGIGETV-RAGRIFKYFAGEALRRHGQNLESTRPGVEIQTYRQAVGVYGLI 146
           +R+ GK L+      + RA    +YFA  A+        +   G    T RQ +GV   I
Sbjct: 90  SRDSGKPLSLARSLDIPRAVSNLRYFAA-AIVPWSSESHAMELGAINYTLRQPLGVVACI 148

Query: 147 TPWNFPIAIPAWKAAPALAFGNTVVIKPAGPTPATANVLADIMAECGAPAGVFNMLFGRG 206
           +PWN P+ +  WK APALA GN VV KP+  TP TA +L ++  E G P GV N++ GRG
Sbjct: 149 SPWNLPLYLFTWKIAPALAAGNAVVAKPSEITPCTAALLGELSIEAGFPPGVLNIVQGRG 208

Query: 207 -SMGDALIKHKDVDGVSFTGSQGVGAQVAAAAVARQARVQLEMGGKNPLIVLDDADLERA 265
             +G AL++H+DV  VSFTGS   GAQ+AAAA  R  ++ LE+GGKNP IV  DADL  A
Sbjct: 209 PEVGQALVEHRDVKAVSFTGSTRTGAQIAAAAAPRFKKLSLELGGKNPAIVFADADLSDA 268

Query: 266 VAIALDGSFFAT-GQRCTASSRLIVQDGIHDKFVALLAEKVAALRVGDALDPNTQIGPAV 324
               +  S FA  G+ C   SRL+VQ  I+D F      KV ALRVGD  +  T +G  V
Sbjct: 269 NLDTIVRSGFANQGEICLCGSRLLVQRSIYDAFRERYLAKVRALRVGDPREAATDLGALV 328

Query: 325 SEDQMETSYRYIDIAASEGGRVVTGGDRIKLDNP---GWYVRPTLIADTQAGMRINNEEV 381
           S +  +     I  A +EGGRV+ GGD + L  P   GWYV PT+I         N +E+
Sbjct: 329 SREHFDKVTGCIAQARAEGGRVLCGGDALALPGPLAGGWYVAPTVIEGLGPETATNQQEI 388

Query: 382 FGPVASTIRVKSYEEALEIANGVEFGLSAGIATTSLKHARHFQRYARAGMTMVNLATAGV 441
           FGPV + I      +AL IANG  +GL+A + TT L  A  F      G+  +N      
Sbjct: 389 FGPVVTLIPFDDEAQALAIANGTGYGLAASLWTTDLSRAHRFGAQLDFGIVWINCWLLR- 447

Query: 442 DYHVPFGGTKSSSYGAREQGFAAVEFFTQTK 472
           D   PFGG K S  G RE G  A+ FFT+ K
Sbjct: 448 DLRTPFGGAKQSGVG-REGGVEALRFFTEPK 477


Lambda     K      H
   0.317    0.133    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 551
Number of extensions: 26
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 478
Length of database: 483
Length adjustment: 34
Effective length of query: 444
Effective length of database: 449
Effective search space:   199356
Effective search space used:   199356
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory