Annotation: FitnessBrowser__HerbieS:HSERO_RS15670
Length: 427 amino acids
Source: HerbieS in FitnessBrowser
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
---|---|---|---|---|---|---|---|---|---|
citrate catabolism | citA | hi | citrate transporter (characterized) | 41% | 95% | 300.8 | alpha-ketoglutarate permease (MHS family) | 36% | 261.2 |
2-oxoglutarate (alpha-ketoglutarate) catabolism | kgtP | lo | alpha-ketoglutarate permease (MHS family) (characterized) | 36% | 95% | 261.2 | Citrate:H+ symporter | 40% | 311.6 |
L-proline catabolism | proP | lo | Ectoine/proline transporter ProP (characterized) | 33% | 84% | 219.2 | Citrate:H+ symporter | 40% | 311.6 |
acetate catabolism | deh | lo | The acetate/monochloroacetate (haloacid) permease, Deh4p (Km = 5.5 mμM for acetate; 9 mμM for monochloroacetate) (characterized) | 33% | 63% | 190.3 | Citrate:H+ symporter | 40% | 311.6 |
View HSERO_RS15670 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
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Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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MSSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLN NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSA RLVLRLR
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory