GapMind for catabolism of small carbon sources

 

Aligments for a candidate for astC in Herbaspirillum seropedicae SmR1

Align Succinylornithine transaminase (EC 2.6.1.81) (characterized)
to candidate HSERO_RS23240 HSERO_RS23240 omega amino acid--pyruvate aminotransferase

Query= reanno::pseudo1_N1B4:Pf1N1B4_3440
         (406 letters)



>lcl|FitnessBrowser__HerbieS:HSERO_RS23240 HSERO_RS23240 omega amino
           acid--pyruvate aminotransferase
          Length = 442

 Score =  152 bits (383), Expect = 2e-41
 Identities = 121/409 (29%), Positives = 180/409 (44%), Gaps = 42/409 (10%)

Query: 20  NYAPAAFIPVRGAGSRVWDQSGRELIDFAGGIAVNVLGHAHPALVAALTEQANKLWHVSN 79
           N+  A  + V   G    D +G  ++D   G+    LGHAHP +V+A+ +    L +  +
Sbjct: 24  NFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVATLDYAPS 83

Query: 80  VFTNEP-ALRLAHKLVDATFAE--RVFFCNSGAEANEAAFKLARRVAHDRFGTEKYEIVA 136
                P A  LA +L   T      VF+  SG+EA + A K+A      +    +  ++ 
Sbjct: 84  FQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGNATRTRLIG 143

Query: 137 ALNSFHGRTLFTVNVGGQSKYSDGFGPKIT-GITHVPYNDLAALKAAV------------ 183
               +HG     ++VGG       F   +  G+ H+P+       A              
Sbjct: 144 RERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEPEYGAHLAD 203

Query: 184 ----------SDKTCAVVLEPIQGEGGVLPAELSYLQGARELCDAHNALLVFDEVQTGMG 233
                     +    AV++EP+ G  GVL     YL+  RE+C  H  LL+FDEV TG G
Sbjct: 204 ELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLIFDEVITGFG 263

Query: 234 RSGKLFAYQHYGVTPDILTSAKSLGGG-FPIAAMLTTEDLAKHLVVGT------HGTTYG 286
           R G  FA  ++ V PDI T+AK L  G  P+ A+    ++    + G       HG TY 
Sbjct: 264 RLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYS 323

Query: 287 GNPLACAVAEAVIDVINTPEVLNGVNAKHDKFKTRLEQIGEKYGL--FTEVRGLGLLLGC 344
           G+PLACA   A ++V  T  VL    A  D F+   E      GL    +VR +GL+ G 
Sbjct: 324 GHPLACAAGLASLEVFQTEGVLENAQAVADYFQ---EAAHALRGLPHVIDVRTIGLIAGI 380

Query: 345 VLSD---AWKGKAKDIFNAAEREGLMILQAGPDVIRFAPSLVVEDADID 390
            L+        +A D F  A  +G++I   G D+I  +P L++    ID
Sbjct: 381 ELASIPGRVGARAYDAFVRAFNDGILIRVTG-DIIALSPPLIINKQQID 428


Lambda     K      H
   0.320    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 443
Number of extensions: 24
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 406
Length of database: 442
Length adjustment: 32
Effective length of query: 374
Effective length of database: 410
Effective search space:   153340
Effective search space used:   153340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory