Align ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 2 (characterized)
to candidate HSERO_RS11445 HSERO_RS11445 ABC transporter permease
Query= reanno::pseudo13_GW456_L13:PfGW456L13_4772 (223 letters) >FitnessBrowser__HerbieS:HSERO_RS11445 Length = 221 Score = 119 bits (298), Expect = 5e-32 Identities = 68/220 (30%), Positives = 123/220 (55%), Gaps = 8/220 (3%) Query: 1 MEFDFSGIIPSLPGLWNGMIMTLKLMAMGVIGGIILGTILALMRLS-HNKVLSNIAGAYV 59 M+ DFSG+ S P L +G ++T+++ A ++ G +LG ++ L RLS +V+ + Y+ Sbjct: 1 MKLDFSGVWESWPSLVHGTLVTVEITAASLVVGCVLGLLVGLGRLSPQRRVIYGLCTIYL 60 Query: 60 NYFRSIPLLLVITWFYLAVPFVLRWITGEDTPIGAFASCIVAFMMFEAAYFCEIVRAGVQ 119 R PLL+ + ++ +P V + A+ I+ ++ AY EIVR +Q Sbjct: 61 TLVRGTPLLVQLFLWFFGLPHV-------GVILPAYVCGILGLGIYSGAYVSEIVRGAIQ 113 Query: 120 SIPKGQMGAAQALGMSYGQMMRLIILPQAFRKMTPLLLQQSIILFQDTSLVYAVGLVDFL 179 SI +GQM AA++LG+ Y MR +ILPQAF +M P L + I L ++++LV + + D + Sbjct: 114 SIERGQMEAARSLGLPYRMAMRRVILPQAFVRMIPPLGNEFIALIKNSALVSLLTIADLM 173 Query: 180 NASRASGDIIGRSNEFLIFAGLVYFIISFAASQLVKRLQK 219 + + RS E + L+Y +++ + +++R++K Sbjct: 174 HEGEKIISVTYRSLETYLAIALIYLLLTSVTTLILRRVEK 213 Lambda K H 0.332 0.144 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 132 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 223 Length of database: 221 Length adjustment: 22 Effective length of query: 201 Effective length of database: 199 Effective search space: 39999 Effective search space used: 39999 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory