GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aatP in Herbaspirillum seropedicae SmR1

Align ABC transporter for L-Asparagine and possibly other L-amino acids, putative ATPase component (characterized)
to candidate HSERO_RS17555 HSERO_RS17555 arginine ABC transporter ATP-binding protein

Query= reanno::pseudo13_GW456_L13:PfGW456L13_4773
         (244 letters)



>lcl|FitnessBrowser__HerbieS:HSERO_RS17555 HSERO_RS17555 arginine
           ABC transporter ATP-binding protein
          Length = 244

 Score =  359 bits (922), Expect = e-104
 Identities = 175/244 (71%), Positives = 207/244 (84%)

Query: 1   MISIKSINKWYGDFQVLTDCSTEVKKGEVIVVCGPSGSGKSTLIKCVNALEPFQKGDIVV 60
           MI+++ ++K+YGDF+VL+ C+  + KGEV+VVCGPSGSGKSTLIK +NALEP Q+G I +
Sbjct: 1   MITLQDVSKYYGDFKVLSACNAVIAKGEVVVVCGPSGSGKSTLIKTINALEPIQEGSITI 60

Query: 61  DGTSIADPKTNLPKLRSRVGMVFQHFELFPHLTITENLTIAQIKVLGRSKEEATKKGLQL 120
           DG  + D  TNLP+LRSRVGMVFQHFELFPHLTI +NLT+AQIKVLGR K+EA + G++ 
Sbjct: 61  DGVKVNDRSTNLPRLRSRVGMVFQHFELFPHLTIIQNLTLAQIKVLGRKKDEAHQHGMKY 120

Query: 121 LERVGLSAHAHKHPGQLSGGQQQRVAIARALAMDPIVMLFDEPTSALDPEMVNEVLDVMV 180
           LERVGL AHA K+PGQLSGGQQQRVAIARALAMDPI MLFDEPTSALDPEMVNEVLDVMV
Sbjct: 121 LERVGLKAHAGKYPGQLSGGQQQRVAIARALAMDPICMLFDEPTSALDPEMVNEVLDVMV 180

Query: 181 QLAHEGMTMMCVTHEMGFARKVADRVIFMDQGKIIEDCKKEEFFGDINARAERTQHFLNK 240
           +LA EGMTM+CVTHEMGFAR VA RVIFMDQG I+ED  K++FF      + R++ FL+K
Sbjct: 181 ELAQEGMTMVCVTHEMGFARMVAHRVIFMDQGSIVEDRSKDDFFAGGGDHSARSRQFLSK 240

Query: 241 ILQH 244
           IL H
Sbjct: 241 ILSH 244


Lambda     K      H
   0.321    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 268
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 244
Length of database: 244
Length adjustment: 24
Effective length of query: 220
Effective length of database: 220
Effective search space:    48400
Effective search space used:    48400
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory