GapMind for catabolism of small carbon sources

 

Aligments for a candidate for bgtA in Herbaspirillum seropedicae SmR1

Align ATPase (characterized, see rationale)
to candidate HSERO_RS19240 HSERO_RS19240 arginine ABC transporter ATP-binding protein

Query= uniprot:Q31RN8
         (261 letters)



>lcl|FitnessBrowser__HerbieS:HSERO_RS19240 HSERO_RS19240 arginine
           ABC transporter ATP-binding protein
          Length = 242

 Score =  254 bits (650), Expect = 9e-73
 Identities = 128/241 (53%), Positives = 172/241 (71%), Gaps = 1/241 (0%)

Query: 21  MIYAEGVEKWYGNQFQALCGVSLTVQRGEVVVMMGPSGSGKSTFLRTLNALESHQRGEIW 80
           MI    V KWYG+ FQ L   S +V++G+VVV+ GPSGSGKST ++T+N LE  Q+G I 
Sbjct: 1   MIELNNVSKWYGS-FQVLTDCSTSVKKGDVVVVCGPSGSGKSTLIKTVNGLEPFQKGTIT 59

Query: 81  IEGHRLSHDRRDIATIRQEVGMVFQQFNLFPHLTVLQNLMLAPVQVRRWPVAQAEATARQ 140
           ++G  +   + +++ +R  +GMVFQ F LFPHL++ +NL +  ++V      +A     +
Sbjct: 60  VDGVSVGDPKTNLSKLRARIGMVFQNFELFPHLSIRENLTIGQIKVLGRSKEEATEKGLK 119

Query: 141 LLERVRIAEQADKYPGQLSGGQQQRVAIARALAMQPRILLFDEPTSALDPEMVREVLDVM 200
            L+RV +    DK+PGQLSGGQQQRVAIARAL+M P  +LFDEPTSALDPEM+ EVLDVM
Sbjct: 120 YLDRVGLLAHKDKFPGQLSGGQQQRVAIARALSMDPIAMLFDEPTSALDPEMINEVLDVM 179

Query: 201 RDLASEGMTMLVATHEVGFAREVADRVVLMADGQIVEEAPPDRFFTAPQSDRAKQFLAQI 260
             LA EGMTM+V THE+GFAR+VA+RVV M  G +VE+   D FF  P+S+RA+ FLA+I
Sbjct: 180 VGLAQEGMTMMVVTHEMGFARKVANRVVFMDKGVVVEDCAKDEFFAQPRSERARDFLAKI 239

Query: 261 L 261
           +
Sbjct: 240 I 240


Lambda     K      H
   0.321    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 188
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 242
Length adjustment: 24
Effective length of query: 237
Effective length of database: 218
Effective search space:    51666
Effective search space used:    51666
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory