Align Fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 (characterized)
to candidate HSERO_RS03355 HSERO_RS03355 fructose-bisphosphate aldolase
Query= SwissProt::Q56815 (354 letters) >FitnessBrowser__HerbieS:HSERO_RS03355 Length = 354 Score = 521 bits (1343), Expect = e-153 Identities = 262/353 (74%), Positives = 300/353 (84%) Query: 1 MALVSMRQLLDHAADDSYGLPAFNVNNMEQVKAIMDAARATSSPVILQGSAGARKYAGEP 60 M LVSMRQLLDHAA++ YGLPAFNVNN+EQV AIM+AA +PVI+Q SAGARKYAGE Sbjct: 1 MPLVSMRQLLDHAAENGYGLPAFNVNNLEQVTAIMEAANEVGAPVIMQASAGARKYAGEA 60 Query: 61 FLRHLIAAAVEAYPEIPVVMHQDHGASPAVCMGAIKSGFSSVMMDGSLKEDGKTPADYDY 120 FLRHLI+AAVEAYP IPVVMHQDHG SPAVCM AIKSGFSSVMMDGSL+ DGKT A Y+Y Sbjct: 61 FLRHLISAAVEAYPHIPVVMHQDHGQSPAVCMAAIKSGFSSVMMDGSLEADGKTVASYEY 120 Query: 121 NVSVTAKVVELAHAVGVSVEGELGCLGSLETGKGEAEDGHGAEEALDHSKLLTDPDEAAQ 180 NV V+ KVVE +HA+GV+VE ELG LGSLET KG+ EDGHGA+ + +LLTD ++AA Sbjct: 121 NVEVSRKVVEFSHAIGVTVEAELGVLGSLETMKGDKEDGHGADGTMTREQLLTDVNQAAD 180 Query: 181 FVKATQCDALAIAIGTSHGAYKFTRKPTGDILAIDRIKAIHQRIPTTHLVMHGSSSVPQE 240 FVKATQCDALAIAIGTSHGAYKF+RKPTGDILAIDRIK IH RIP THLVMHGSSSVPQE Sbjct: 181 FVKATQCDALAIAIGTSHGAYKFSRKPTGDILAIDRIKEIHARIPNTHLVMHGSSSVPQE 240 Query: 241 LLEEIRTYGGDIKETYGVPVEEIQEGIRYGVRKVNIDTDIRLAMTAAIRRVGAKNKSEFD 300 LL EIR +GGD+KETYGVPVEEIQEGI++GVRK+NIDTDIRLAMT AIRR +N S+FD Sbjct: 241 LLAEIREFGGDMKETYGVPVEEIQEGIKHGVRKINIDTDIRLAMTGAIRRYLFENPSKFD 300 Query: 301 PRKFMAAAMEEAKKVCIARFEAFGSAGKAEKIRAIELDEMAKRYASGELAQVV 353 PR ++ A E AK VC ARF +FG G+A KI+ I L+++A++Y SGELAQ+V Sbjct: 301 PRDYLKPAREAAKLVCKARFLSFGCEGQAGKIKPISLEKIAEKYKSGELAQIV 353 Lambda K H 0.316 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 463 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 354 Length of database: 354 Length adjustment: 29 Effective length of query: 325 Effective length of database: 325 Effective search space: 105625 Effective search space used: 105625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
Align candidate HSERO_RS03355 HSERO_RS03355 (fructose-bisphosphate aldolase)
to HMM TIGR01521 (fba: fructose-bisphosphate aldolase, class II, Calvin cycle subtype (EC 4.1.2.13))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01521.hmm # target sequence database: /tmp/gapView.32555.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01521 [M=347] Accession: TIGR01521 Description: FruBisAldo_II_B: fructose-bisphosphate aldolase, class II, Calvin cycle subtype Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.1e-181 588.6 0.9 2.3e-181 588.4 0.9 1.0 1 lcl|FitnessBrowser__HerbieS:HSERO_RS03355 HSERO_RS03355 fructose-bisphosph Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__HerbieS:HSERO_RS03355 HSERO_RS03355 fructose-bisphosphate aldolase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 588.4 0.9 2.3e-181 2.3e-181 1 347 [] 3 349 .. 3 349 .. 1.00 Alignments for each domain: == domain 1 score: 588.4 bits; conditional E-value: 2.3e-181 TIGR01521 1 lislrqlldhaaergygvpafnvnnleqilaimeaadktdspvilqasrgarsyagevllrklvlaav 68 l+s+rqlldhaae+gyg+pafnvnnleq+ aimeaa++ +pvi+qas+gar+yage++lr+l+ aav lcl|FitnessBrowser__HerbieS:HSERO_RS03355 3 LVSMRQLLDHAAENGYGLPAFNVNNLEQVTAIMEAANEVGAPVIMQASAGARKYAGEAFLRHLISAAV 70 69****************************************************************** PP TIGR01521 69 eeypdipvvlhqdhgnspatclsaiqlgftsvmmdgslkedaktpadydynvsvtaevvklahavgas 136 e yp+ipvv+hqdhg+spa+c++ai+ gf+svmmdgsl++d+kt a+y+ynv+v+ +vv+++ha+g++ lcl|FitnessBrowser__HerbieS:HSERO_RS03355 71 EAYPHIPVVMHQDHGQSPAVCMAAIKSGFSSVMMDGSLEADGKTVASYEYNVEVSRKVVEFSHAIGVT 138 ******************************************************************** PP TIGR01521 137 vegelgclgsletgkgeaedghgfegaldrsqlltdpeeaaefvkktkvdalavaigtshgaykftrk 204 ve elg lgslet kg++edghg++g++ r qlltd ++aa+fvk t+ dala+aigtshgaykf+rk lcl|FitnessBrowser__HerbieS:HSERO_RS03355 139 VEAELGVLGSLETMKGDKEDGHGADGTMTREQLLTDVNQAADFVKATQCDALAIAIGTSHGAYKFSRK 206 ******************************************************************** PP TIGR01521 205 ptgevlaidrieeiherlpdthlvmhgsssvpqewldvineyggeiketygvpveeivkgikfgvrkv 272 ptg++laidri+eih+r+p+thlvmhgsssvpqe l+ i e+gg++ketygvpveei++gik+gvrk+ lcl|FitnessBrowser__HerbieS:HSERO_RS03355 207 PTGDILAIDRIKEIHARIPNTHLVMHGSSSVPQELLAEIREFGGDMKETYGVPVEEIQEGIKHGVRKI 274 ******************************************************************** PP TIGR01521 273 nidtdlrlaataalrrvaakdpsefdprkflkkaveamkdvckaryeafgtagnaskikvvsleemar 340 nidtd rla+t+a+rr + ++ps+fdpr +lk+a ea k vckar+ +fg g+a kik++sle++a+ lcl|FitnessBrowser__HerbieS:HSERO_RS03355 275 NIDTDIRLAMTGAIRRYLFENPSKFDPRDYLKPAREAAKLVCKARFLSFGCEGQAGKIKPISLEKIAE 342 ******************************************************************** PP TIGR01521 341 ryakgel 347 +y +gel lcl|FitnessBrowser__HerbieS:HSERO_RS03355 343 KYKSGEL 349 *****97 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (347 nodes) Target sequences: 1 (354 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.16 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory