Alignments for a candidate for HSERO_RS05250 in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS05250 in Herbaspirillum seropedicae SmR1

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate HSERO_RS03640 HSERO_RS03640 D-ribose transporter ATP-binding protein

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__HerbieS:HSERO_RS03640
          Length = 502

 Score =  434 bits (1117), Expect = e-126
 Identities = 236/499 (47%), Positives = 330/499 (66%), Gaps = 6/499 (1%)

Query: 18  SSVPVIALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSG 77
           +  P++ +R + K F   LAL +    +  GE+HALMGENGAGKSTLMK+LSGV+  D G
Sbjct: 6   TQTPLLQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQG 65

Query: 78  DILLDGKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDEL 137
           +ILLDG+PV + +P  ++A GI +I+QEL +  ++S A N+F+G E R  +GL ID   +
Sbjct: 66  EILLDGRPVALRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGL-IDHAAM 124

Query: 138 NRQAAAIFARMRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIA 197
             +  A+  ++      S   G L++A QQ VEIA+AL   SR++IMDEPTAAL+  E  
Sbjct: 125 RSRTDAVLRQLGAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETE 184

Query: 198 ELFRIIRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMV 257
           +LF ++R L+ +G+ I+YISH+M E+  +ADRV+V+RDG ++  +   E   + I+ MMV
Sbjct: 185 QLFNVVRRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMV 244

Query: 258 GRALDG---EQRIPP-DTSRNDVVLEVRGLNRGRAIRDVSFTLRKGEILGFAGLMGAGRT 313
           GR+L      QRI P D ++   V++VR L  G+ IR  SF +R GE+LGFAGL+GAGRT
Sbjct: 245 GRSLSEFYQHQRIAPADAAQLPTVMQVRALAGGK-IRPASFDVRAGEVLGFAGLVGAGRT 303

Query: 314 EVARAIFGADPLEAGEIIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIA 373
           E+AR +FGADP   G+I++ G    I  P  A+  GI Y+ EDRK  GL + M V AN  
Sbjct: 304 ELARLLFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANAT 363

Query: 374 LSSMGRFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRD 433
           ++   R TR+G +  R++   A+  +++L +K    E     LSGGNQQK+++A+WL   
Sbjct: 364 MNVASRHTRLGLVRSRSLGGVARAAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIA 423

Query: 434 CDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRI 493
             +L  DEPTRG+D+ AKSEIY+L+  LA QG A+V+ISSELPEV+ +  RVLVM EG I
Sbjct: 424 PKVLILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMI 483

Query: 494 TGELARADATQEKIMQLAT 512
           TGELA A  TQE IM+LAT
Sbjct: 484 TGELAGAAITQENIMRLAT 502



 Score =  112 bits (281), Expect = 2e-29
 Identities = 74/252 (29%), Positives = 131/252 (51%), Gaps = 10/252 (3%)

Query: 267 IPPDTSRNDVVLEVRGLNRG----RAIRDVSFTLRKGEILGFAGLMGAGRTEVARAIFGA 322
           IP D ++  + L++RG+ +      A+ D+  T+R GEI    G  GAG++ + + + G 
Sbjct: 1   IPVDMTQTPL-LQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGV 59

Query: 323 DPLEAGEIIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIALSSMGRFTR 382
              + GEI++ G    ++ P  + A GI  + ++     +A  + V AN+ + S  R TR
Sbjct: 60  HAPDQGEILLDGRPVALRDPGASRAAGINLIYQE---LAVAPNISVAANVFMGSELR-TR 115

Query: 383 VGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRDCDILFFDEP 442
           +G +D  A+R      +RQL     + +   RL S   QQ++ IA+ L+    I+  DEP
Sbjct: 116 LGLIDHAAMRSRTDAVLRQLGAGFGASDLAGRL-SIAEQQQVEIARALVHRSRIVIMDEP 174

Query: 443 TRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRITGELARADA 502
           T  +      +++ ++  L ++G AI+ IS  + EV  ++ RV V+ +G   GEL R + 
Sbjct: 175 TAALSERETEQLFNVVRRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEI 234

Query: 503 TQEKIMQLATQR 514
             E+I+Q+   R
Sbjct: 235 DSERIVQMMVGR 246


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 698
Number of extensions: 35
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 520
Length of database: 502
Length adjustment: 35
Effective length of query: 485
Effective length of database: 467
Effective search space:   226495
Effective search space used:   226495
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources