Alignments for a candidate for HSERO_RS05250 in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS05250 in Herbaspirillum seropedicae SmR1

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate HSERO_RS11485 HSERO_RS11485 ribose ABC transporter ATPase

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__HerbieS:HSERO_RS11485
          Length = 522

 Score =  396 bits (1018), Expect = e-115
 Identities = 227/504 (45%), Positives = 317/504 (62%), Gaps = 15/504 (2%)

Query: 15  SSSSSVPVIALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQR 74
           S S   P++ L  + KR+   + LD    +L  G+V AL GENGAGKSTL KI+ G+   
Sbjct: 6   SLSQGSPLLTLSGIGKRYAAPV-LDGIDLDLRPGQVLALTGENGAGKSTLSKIICGLVDA 64

Query: 75  DSGDILLDGKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDE 134
            +G ++LDG+P       QA+ LGI ++ QELNL+  LS A+N+F+ + PR+    +ID 
Sbjct: 65  SAGGMMLDGQPYAPASRTQAEGLGIRMVMQELNLIPTLSIAENLFLEKLPRRFG--WIDR 122

Query: 135 DELNRQAAAIFARMRL-DMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNN 193
            +L   A A    + L ++DP TPVG+L +  QQMVEIA+ L    R LI+DEPTA L N
Sbjct: 123 KKLAEAARAQMEVVGLGELDPWTPVGDLGLGHQQMVEIARNLIGSCRCLILDEPTAMLTN 182

Query: 194 AEIAELFRIIRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTII 253
            E+  LF  I  L+A+GV I+YISH+++EL++IADR+ V+RDGK +    +   S + ++
Sbjct: 183 REVELLFSRIERLRAEGVAIIYISHRLEELKRIADRIVVLRDGKLVCNDDIGRYSTEQLV 242

Query: 254 SMMVGRA----LDGEQRIPPDTSRNDVVLEVRGLNRGRAIRDVSFTLRKGEILGFAGLMG 309
            +M G      LD E R          VL +RGL R   +   S  L  GE+LG AGL+G
Sbjct: 243 QLMAGELTKVDLDAEHR-----RIGAPVLRIRGLGRAPVVHPASLALHAGEVLGIAGLIG 297

Query: 310 AGRTEVARAIFGADPLEAGEIIIHGGK--AVIKSPADAVAHGIGYLSEDRKHFGLAVGMD 367
           +GRTE+ R IFGAD  E GEI I   +  A I+SP DAV  GI  ++EDRK  GL +   
Sbjct: 298 SGRTELLRLIFGADRAEQGEIFIGDSQEPARIRSPKDAVKAGIAMVTEDRKGQGLLLPQA 357

Query: 368 VQANIALSSMGRFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIA 427
           +  N +L+++G  +R G +D  A    AQ YV++L I++ SV Q A  LSGGNQQK+VIA
Sbjct: 358 ISVNTSLANLGSVSRGGMLDHAAESSVAQDYVKKLRIRSGSVAQAAGELSGGNQQKVVIA 417

Query: 428 KWLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLV 487
           +WL RDC I+ FDEPTRGID+GAKS+IY+L   LA QGK ++++SS+L E++++  R+ V
Sbjct: 418 RWLYRDCPIMLFDEPTRGIDIGAKSDIYRLFAELAAQGKGLLVVSSDLRELMQICDRIAV 477

Query: 488 MCEGRITGELARADATQEKIMQLA 511
           M  GRI    +R D +QE+I+  A
Sbjct: 478 MSAGRIADTFSRDDWSQERILAAA 501


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 636
Number of extensions: 24
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 522
Length adjustment: 35
Effective length of query: 485
Effective length of database: 487
Effective search space:   236195
Effective search space used:   236195
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources