Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate HSERO_RS08285 HSERO_RS08285 amino acid ABC transporter ATP-binding protein
Query= uniprot:Q1MCU2 (292 letters) >FitnessBrowser__HerbieS:HSERO_RS08285 Length = 258 Score = 249 bits (637), Expect = 3e-71 Identities = 130/261 (49%), Positives = 181/261 (69%), Gaps = 9/261 (3%) Query: 13 TLLKVEHLSMKFGGLMAINDFSFEAKRGDITALIGPNGAGKTTVFNCITGFYKPTMGMIT 72 T+L+V +LSM+FGGL+A++ S + ++ A+IGPNGAGKTTVFNCI+GFY+PT G I Sbjct: 7 TMLEVSNLSMRFGGLLAVDGVSLAVQEREVFAIIGPNGAGKTTVFNCISGFYRPTSGEIM 66 Query: 73 FNQKSGKQYLLERLPDFRITKEARVARTFQNIRLFSGLTVLENLLVAQHNKLMKASGYTI 132 + S + R P ++ ++ V RTFQNIRLF +TV+ENLLVAQH ++ + + Sbjct: 67 LDGAS-----IARQPSHQVAQQGLV-RTFQNIRLFKSMTVVENLLVAQHQQV---NTNLL 117 Query: 133 LGLIGVGPYKREAAEAIELARFWLEKADLIDRADDPAGDLPYGAQRRLEIARAMCTGPEL 192 GL+ Y++ EA++ A +WL++ L A+ AG L YG QRR+EIAR M T P L Sbjct: 118 SGLLKTPAYRKSEQEALQRAAYWLDQLGLTALANREAGTLSYGLQRRVEIARCMITKPRL 177 Query: 193 LCLDEPAAGLNPRESATLNALLKSIRAETGTSILLIEHDMSVVMEISDHVVVLEYGQKIS 252 L LDEPAAGLNP+E L+ L+ +R E G ++LLIEHDMS++M ISD ++V+E+G+ I Sbjct: 178 LLLDEPAAGLNPQEKQELSQLIDRLRREHGVAVLLIEHDMSLIMGISDRILVMEHGKPIV 237 Query: 253 DGTPDHVKNDPRVIAAYLGVE 273 GTP+ V++D RVI AYLG E Sbjct: 238 TGTPEQVRSDERVIKAYLGEE 258 Lambda K H 0.318 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 202 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 292 Length of database: 258 Length adjustment: 25 Effective length of query: 267 Effective length of database: 233 Effective search space: 62211 Effective search space used: 62211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory