Alignments for a candidate for glpV in Herbaspirillum seropedicae SmR1

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpV in Herbaspirillum seropedicae SmR1

Align ABC transporter for Glycerol, periplasmic substrate-binding component (characterized)
to candidate HSERO_RS04870 HSERO_RS04870 ABC transporter substrate-binding protein

Query= reanno::acidovorax_3H11:Ac3H11_796
         (577 letters)



>FitnessBrowser__HerbieS:HSERO_RS04870
          Length = 582

 Score =  955 bits (2469), Expect = 0.0
 Identities = 451/576 (78%), Positives = 501/576 (86%), Gaps = 2/576 (0%)

Query: 4   QFKAIAFAAAALAMGQA--AWAGEAEAKKWIDSEFQPSTLNKDQQMAEMKWFIDAAKKLQ 61
           + K    AAA +  G A  AWA    A+KW+DSEFQPSTL++ QQ++EM+WFIDAA KL+
Sbjct: 7   KIKLTVLAAAMVMAGVAGNAWADIKAAEKWVDSEFQPSTLSRPQQVSEMQWFIDAAAKLK 66

Query: 62  AKGVKEISVVSETITTHEYESKTLAKAFEEITGIKVKHDLIQEGDVVEKLQTSMQSGKSI 121
           AKGVKEI VVSET+ TH YESKTLAKAFEEITGIKV HD+IQEGDVVEK+QTS+QSGKSI
Sbjct: 67  AKGVKEIHVVSETLDTHAYESKTLAKAFEEITGIKVIHDVIQEGDVVEKMQTSLQSGKSI 126

Query: 122 YDGWISDSDLIGTHYRYGKIMNLTDYMGGAGKEWTNPGIDLKDYIGTKFTTGPDGKLYQL 181
           YDGW++DSDLIGTHYR G+ + LTDYM G GKE+TNPG+DLKD+IGT FTT PDGKLYQL
Sbjct: 127 YDGWVNDSDLIGTHYRNGQTVVLTDYMAGPGKEYTNPGLDLKDFIGTSFTTAPDGKLYQL 186

Query: 182 PDQQFANLYWFRADLFDRKDIKDKFKAKYGYDLGVPLNWSAYEDIAEFFTNDVKNIDGKP 241
           PDQQFANLYWFRAD F RKD+++KFKAKYGY+LGVP NWSAYEDIA FFTNDVK +DGK 
Sbjct: 187 PDQQFANLYWFRADWFARKDLQEKFKAKYGYELGVPQNWSAYEDIAAFFTNDVKELDGKK 246

Query: 242 IYGHMDYGKKDPSLGWRFTDAWLSMAGTADIGAPNGLPIDEWGIRVADDKCTPVGASVAR 301
           IYGHMDYGKKDPSLGWRFTDAWLSMAG AD G PNGLP+DEWGIRVADDKCTPVGASVAR
Sbjct: 247 IYGHMDYGKKDPSLGWRFTDAWLSMAGAADKGLPNGLPVDEWGIRVADDKCTPVGASVAR 306

Query: 302 GGATNSPAAVYALTKYVDWMKKYAPKEATGMTFGEAGPVPAQGQIAQQIFWYTAFTADMT 361
           GGATNSPAAVYALTKY+DWMKKYAP EA GMTF EAGPVPAQG IAQQIFWY+AFTA MT
Sbjct: 307 GGATNSPAAVYALTKYLDWMKKYAPPEALGMTFSEAGPVPAQGHIAQQIFWYSAFTASMT 366

Query: 362 KPGLPVVNADGTPKWRMAPGPNGPYWKQGMQNGYQDVGSWTFFKDHDANKTAAAWLYAQF 421
           K GLPVVNADG+PKWRMAPGP+GPYWK GMQNGYQDVGSWTF K    ++ AAAWLYAQF
Sbjct: 367 KQGLPVVNADGSPKWRMAPGPHGPYWKDGMQNGYQDVGSWTFLKSTPPDQMAAAWLYAQF 426

Query: 422 VTAKTTSLKKTMVGLTPIRESDIQSKAMTDMAPKLGGLVEFYRSPARVAWSPTGTNVPDY 481
            T+KT SLKK++VG+T IR+SDI+S   T  A K GGL+EFYRSPARVAW+PTGTNVPDY
Sbjct: 427 TTSKTVSLKKSIVGVTFIRDSDIRSDYFTKNAAKYGGLIEFYRSPARVAWTPTGTNVPDY 486

Query: 482 PKLAQLWWKNVAQAVTGEKTPQGAMDTLADEMDQVMARLERAGMAHCAPKLNAKSDPNKW 541
           PK+AQ+WWKN++ A++GEKTPQGAMD LADEMD +MARL+RAGM  CAPKLN K DP  W
Sbjct: 487 PKMAQIWWKNISSAISGEKTPQGAMDNLADEMDGIMARLQRAGMKQCAPKLNPKKDPATW 546

Query: 542 LSDKQAPWKKLANEKPKGETIAYGTLLQAWKDGKTR 577
           LSDK APW KLANEKPKGETI Y  LLQAWKDG+ R
Sbjct: 547 LSDKGAPWAKLANEKPKGETIPYDKLLQAWKDGRVR 582


Lambda     K      H
   0.315    0.132    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1448
Number of extensions: 84
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 577
Length of database: 582
Length adjustment: 36
Effective length of query: 541
Effective length of database: 546
Effective search space:   295386
Effective search space used:   295386
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources