Align Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; Beta-ketothiolase; EC 2.3.1.9 (characterized)
to candidate HSERO_RS01180 HSERO_RS01180 acetyl-CoA acetyltransferase
Query= SwissProt::P14611 (393 letters) >FitnessBrowser__HerbieS:HSERO_RS01180 Length = 391 Score = 591 bits (1524), Expect = e-173 Identities = 298/392 (76%), Positives = 339/392 (86%), Gaps = 1/392 (0%) Query: 1 MTDVVIVSAARTAVGKFGGSLAKIPAPELGAVVIKAALERAGVKPEQVSEVIMGQVLTAG 60 M DVVIV+A RTA+GKFGG+L+KI A +LGA VIKA L + G+KPE +SEVI+GQVLTAG Sbjct: 1 MDDVVIVAAQRTAIGKFGGALSKIAAADLGAQVIKALLAKTGIKPEAISEVILGQVLTAG 60 Query: 61 SGQNPARQAAIKAGLPAMVPAMTINKVCGSGLKAVMLAANAIMAGDAEIVVAGGQENMSA 120 GQNPARQ+ IK+GLP MVPA + KVCGSGLKAV LAA AI GDA+IV+AGGQENMSA Sbjct: 61 LGQNPARQSVIKSGLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQENMSA 120 Query: 121 APHVLPGSRDGFRMGDAKLVDTMIVDGLWDVYNQYHMGITAENVAKEYGITREAQDEFAV 180 +PHVL SRDGFRMGDAKL DTMIVDGLWDVYNQYHMGITAENVAK++ I+RE QD FA Sbjct: 121 SPHVLNNSRDGFRMGDAKLTDTMIVDGLWDVYNQYHMGITAENVAKKFEISREEQDAFAA 180 Query: 181 GSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEFVRQGATLDSMSGLKPAFDKAG 240 SQNKAEAAQKAGKF +EIVP+ I +K + V F TDEFV+ G T +S++ L+PAFDKAG Sbjct: 181 ASQNKAEAAQKAGKFKDEIVPIEIKGKK-ETVVFDTDEFVKHGVTAESLATLRPAFDKAG 239 Query: 241 TVTAANASGLNDGAAAVVVMSAAKAKELGLTPLATIKSYANAGVDPKVMGMGPVPASKRA 300 TVTA NASG+NDGAAAVVV SA A ELGL LA IK+Y++AG+DP +MGMGPVPAS+ Sbjct: 240 TVTAGNASGINDGAAAVVVTSAKLAAELGLPVLAKIKAYSSAGLDPSIMGMGPVPASQLT 299 Query: 301 LSRAEWTPQDLDLMEINEAFAAQALAVHQQMGWDTSKVNVNGGAIAIGHPIGASGCRILV 360 L +A WTPQDLDLMEINEAFAAQA+AV++QMGWDTSK+NVNGGAIA+GHPIGASGCR+LV Sbjct: 300 LKKAGWTPQDLDLMEINEAFAAQAIAVNKQMGWDTSKINVNGGAIALGHPIGASGCRVLV 359 Query: 361 TLLHEMKRRDAKKGLASLCIGGGMGVALAVER 392 TLLHEM RRDAKKGLASLCIGGGMGVALAVER Sbjct: 360 TLLHEMVRRDAKKGLASLCIGGGMGVALAVER 391 Lambda K H 0.315 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 529 Number of extensions: 18 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 393 Length of database: 391 Length adjustment: 31 Effective length of query: 362 Effective length of database: 360 Effective search space: 130320 Effective search space used: 130320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate HSERO_RS01180 HSERO_RS01180 (acetyl-CoA acetyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.29264.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-152 492.9 10.6 3.5e-152 492.7 10.6 1.0 1 lcl|FitnessBrowser__HerbieS:HSERO_RS01180 HSERO_RS01180 acetyl-CoA acetylt Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__HerbieS:HSERO_RS01180 HSERO_RS01180 acetyl-CoA acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 492.7 10.6 3.5e-152 3.5e-152 1 385 [] 6 390 .. 6 390 .. 0.99 Alignments for each domain: == domain 1 score: 492.7 bits; conditional E-value: 3.5e-152 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaa 68 iv+a Rt+igk+gg+l+++ a+dL+a+vik+ll+++g++pe+i evilG+vl+ag ++n+aR+ ++++ lcl|FitnessBrowser__HerbieS:HSERO_RS01180 6 IVAAQRTAIGKFGGALSKIAAADLGAQVIKALLAKTGIKPEAISEVILGQVLTAGLGQNPARQSVIKS 73 8******************************************************************* PP TIGR01930 69 glpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakl 136 glp+ vpa+ v +vC+Sgl+Av+laaq+i++G+a++v+aGG E+mS +p++l++s r+++++g+akl lcl|FitnessBrowser__HerbieS:HSERO_RS01180 74 GLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQENMSASPHVLNNS--RDGFRMGDAKL 139 *****************************************************98..9********** PP TIGR01930 137 edqllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk 201 d+++ d+ v+++++mg+tAen+akk++isReeqD++a++S++ka++A+++gkfkdeivp+e+kgk lcl|FitnessBrowser__HerbieS:HSERO_RS01180 140 TDTMIVDGlwdVYNQYHMGITAENVAKKFEISREEQDAFAAASQNKAEAAQKAGKFKDEIVPIEIKGK 207 *******9***99******************************************************9 PP TIGR01930 202 ..kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltpla 267 ++v+++De ++ ++t+e+La+L+paf++ +g tvtAgN+s++nDGAaa++++s + a+elgl +la lcl|FitnessBrowser__HerbieS:HSERO_RS01180 208 keTVVFDTDEFVKHGVTAESLATLRPAFDK-AG-TVTAGNASGINDGAAAVVVTSAKLAAELGLPVLA 273 99999***********************95.9*.6********************************* PP TIGR01930 268 rivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvn 335 +i +++ ag+dp++mg+gpvpA++ Lkkag++ +d+dl+EinEAFAaq++av+k++g d++k+Nvn lcl|FitnessBrowser__HerbieS:HSERO_RS01180 274 KIKAYSSAGLDPSIMGMGPVPASQLTLKKAGWTPQDLDLMEINEAFAAQAIAVNKQMG-WDTSKINVN 340 **********************************************************.88******* PP TIGR01930 336 GGAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385 GGAiAlGHP+GasG+r+++tll+e+ +r++k Gla+lC+ggG+G+A+ +e lcl|FitnessBrowser__HerbieS:HSERO_RS01180 341 GGAIALGHPIGASGCRVLVTLLHEMVRRDAKKGLASLCIGGGMGVALAVE 390 **********************************************9886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (391 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.49 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory