Align GDP-6-deoxy-D-talose 4-dehydrogenase (EC 1.1.1.135); 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC 1.1.1.178) (characterized)
to candidate HSERO_RS05565 HSERO_RS05565 short-chain dehydrogenase
Query= BRENDA::Q99714 (261 letters) >FitnessBrowser__HerbieS:HSERO_RS05565 Length = 249 Score = 125 bits (314), Expect = 8e-34 Identities = 89/226 (39%), Positives = 126/226 (55%), Gaps = 18/226 (7%) Query: 8 VKGLVAVITGGA--SGLGLATAERLVGQGASAVLLDLPNSGGEAQAKKLGNNCVFAPADV 65 +K VAVITGGA +GLG ATA + QGA V+LDL + A A ++G+ + ADV Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADV 62 Query: 66 TSEKDVQTALALAKGKFGRVDVAVNCAGIAVASKTYNLKKGQTHTLEDFQRVLDVNLMGT 125 T + +TA A +FGR+D+ VN AGI KT + T D+ R+LDV+L GT Sbjct: 63 TDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEI------TGADYDRILDVSLRGT 116 Query: 126 FNVIRLVAGEMGQNEPDQGGQRGVIINTASVAAFEGQ--VGQAAYSASKGGIVGMTLPIA 183 + + V M + Q G II +SV+A G +G YSA+K G++G+ +A Sbjct: 117 LYMSQAVLPAM------RAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMA 170 Query: 184 RDLAPIGIRVMTIAPGLFGTPLLT-SLPEKVCNFLASQVPFPSRLG 228 R+ P GIRV +I PGL GT ++ L E+ +A +P +RLG Sbjct: 171 REFGPEGIRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPL-ARLG 215 Lambda K H 0.318 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 155 Number of extensions: 5 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 261 Length of database: 249 Length adjustment: 24 Effective length of query: 237 Effective length of database: 225 Effective search space: 53325 Effective search space used: 53325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory